Application description
This method details the High-Performance Liquid Chromatography (HPLC) analysis of six preservatives: benzyl alcohol, dehydroacetic acid, sorbic acid, methylparaben, benzoic acid, and ethylparaben. The separation is conducted on an Amaze HA Mixed-Mode Column, utilizing both reversed-phase and anion-exchange modes for comprehensive separation.
Benzyl Alcohol: A commonly used preservative with antimicrobial properties, benzyl alcohol is a water-miscible compound with a phenyl group. It is often employed in pharmaceutical formulations and cosmetic products.
Dehydroacetic Acid: Another antimicrobial preservative, dehydroacetic acid is water-soluble and contains carboxylic acid functional groups. It is frequently utilized in the preservation of food, cosmetics, and personal care products.
Sorbic Acid: Widely employed as a preservative in food and beverages, sorbic acid is water-soluble and possesses a carboxylic acid group. It inhibits the growth of mold, yeast, and fungi in various formulations.
Methylparaben: Methylparaben is an ester of p-hydroxybenzoic acid, serving as a preservative in pharmaceuticals, cosmetics, and food products. It is soluble in alcohol and exhibits antimicrobial activity against a range of microorganisms.
Benzoic Acid: A naturally occurring compound with antimicrobial properties, benzoic acid is soluble in water and commonly used as a preservative in acidic food products, beverages, and personal care items.
Ethylparaben: Ethylparaben is an ester of p-hydroxybenzoic acid, similar to methylparaben, and is utilized as a preservative in various consumer products. It exhibits solubility in alcohol and serves to extend the shelf life of formulations.
Amaze HA Mixed-Mode Column: This column features a mixed-mode stationary phase with both hydrophobic and ionic interaction capabilities. In reversed-phase mode, hydrophobic interactions predominate, facilitating separation based on differences in hydrophobicity. In anion-exchange mode, ionic interactions occur, allowing for separation based on differences in charge.
By employing this method, comprehensive analysis and quantification of the six preservatives can be achieved, making it valuable for quality control and regulatory compliance in various industries.
Conditions of Experiment
| Column: |
Amaze HA |
| Separation Modes: |
reversed-phase, Anion-exchange |
| Column Dimenstions: |
3x100 mm, 3 um, 100 A |
| Mobile Phase: |
ACN/water/ammonium formate pH 3.7 |
| Detection: |
235 nm |
| Sample: |
various-0.2 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
0.6 ml/min |
Analytes
| Class of compounds: |
Aromatic acid, Aromatic compound, Organic acid, Organic compound, Preservative |
| Nature of compounds: |
Acidic, Hydrophilic, Hydrophobic, Neutral |
| Compounds: |
Benzyl alcohol, Dehydroacetic acid, Sorbic acid, Methylparaben, Benzoic acid, Ethylparaben |
