Bromate Ion

Organic and inorganic acids are part of many formulations including pharmaceutical and nutraceuticals. These compounds are very often very polar and acidic, and either require expensive ion-chromatography or the use of ion-pairing reagents. Ion-pairing reagents are not compatible with mass spectrometry. Mixed-mode columns allow the utilization of the ion-pairing reagent attached to the surface of silica gel to retain these anionic compounds. The retention is provided by weak reverse-phase and by a medium or strong anion-exchange mechanism. The retention time is controlled by the amount of ACN, buffer pH and buffer concentration. Stronger and more hydrophobic acids will retain longer, and their elution can be effectively controlled by changing the ionization state at a different pH. This method is compatible with major detection techniques ( (ELSD, CAD, RI, UV and mass spec). This method can be used for the analysis of basic hydrophobic drugs and their acidic counter-ions in one run. Acidic hydrophobic drugs and related impurities can be analyzed on this mixed-mode HPLC column.

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Conditions of Experiment

Column:	Heritage MA
Separation Modes:	anion-exchange
Column Dimenstions:	4.6x150 mm, 3 um, 100A
Mobile Phase:	60% ACN with 60 mM AmFm pH 3
Detection:	ELSD, 50°C
Sample:	1 mg/ml
Injection:	3 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Inorganic anion, Organic acid
Nature of compounds:	Acidic, Hydrophilic, Polar
Compounds:	Phosphate Ion, Bromate Ion, Bromide ion, Chloride Ion, Iodide ion, Trifluoroacetic acid, Perchlorate ion

Chlorate and bromate are polar inorganic acidic ions. We developed a separation approach on the Heritage MA column for the separation of these two anions. Both compounds are retained by a pure ion-exchange mechanism. The retention time can be adjusted by the buffer pH and buffer concentrations. These and other ions can be analyzed in one run in an isocratic or gradient mode. This method is compatible with MS, ELSD, CAD and RI detection techniques. This method can be used for the analysis of inorganic and inorganic anions in various matrices. Basic drugs and counterions can be separated in one run on this column. The basic drug needs to be hydrophobic enough to compensate for the presence of a cation-exclusion mechanism on the Heritage MA column.

Heritage MA PURCHASE LEARN ABOUT Heritage MA

Conditions of Experiment

Column:	Heritage MA
Separation Modes:	anion-exchange
Column Dimenstions:	4.6x150 mm, 3 um, 100A
Mobile Phase:	60% ACN with 60 mM AmFm pH 3
Detection:	ELSD, 50°C
Sample:	1 mg/ml
Injection:	3 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Inorganic anion, Organic acid
Nature of compounds:	Acidic, Hydrophilic, Polar
Compounds:	Bromate Ion, Chlorate ion