Bromide ion

Most of the over-the-counter cold and cough remedies contain a complex mixture of basic, acidic, neutral, hydrophilic, and hydrophobic compounds. Analysis of drugs and counterions usually require two methods - one for the active drug and related impurities and another one for analysis of counterions. The method can be simplified by switching to mixed-mode stationary phases. Mixed-mode stationary phases have ion-pairing reagent attached to the surface of silica gel. The presence of ionizable groups on the surface allows us to achieve 2-D separation on a single column. We have developed a reliable, robust high-efficiency method for analysis of active and inactive ingredients of Mucinex on Heritage MA mixed-mode column. The active ingredients of the formulation include guaifenesin and dextromethorphan. Guaifenesin is a hydrophilic organic compound that is used as a drug that is used to help to remove mucus from the mouth due to the common cold and flu. Dextromethorphan is a hydrophobic basic compound, which is used as an over-the-counter cough suppressant. Excipients are hydrophilic and hydrophobic in nature and are added to improve taste and increase the stability of the formulation. Heritage MA column shows exceptional peak shape and does not demonstrate poor peak shape for basic analytes like dextromethorphan. Heritage MA column can be used for analysis and validation of methods for separation of hydrophobic, hydrophobic basic, hydrophobic acidic and hydrophilic acidic compounds. There are several mechanisms available to be used with this column - reversed-phase, anion-exchange, cation-exclusion, and combination of the above.

Heritage MA PURCHASE LEARN ABOUT Heritage MA

Conditions of Experiment

Column:	Heritage MA
Separation Modes:	reversed-phase, cation-exclusion, anion-exchange
Column Dimenstions:	4.6x150 mm, 3 um, 100A
Mobile Phase:	8% ACN with 30 mM NaH2PO4 pH 3.1
Detection:	UV 255
Sample:	various
Injection:	3 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Amines, Aromatic base, Aromatic compound, Drug, Inorganic anion, Organic acid
Nature of compounds:	Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds:	Bromide ion, Dextromethorphan, Guaifenesin

Organic and inorganic acids are part of many formulations including pharmaceutical and nutraceuticals. These compounds are very often very polar and acidic, and either require expensive ion-chromatography or the use of ion-pairing reagents. Ion-pairing reagents are not compatible with mass spectrometry. Mixed-mode columns allow the utilization of the ion-pairing reagent attached to the surface of silica gel to retain these anionic compounds. The retention is provided by weak reverse-phase and by a medium or strong anion-exchange mechanism. The retention time is controlled by the amount of ACN, buffer pH and buffer concentration. Stronger and more hydrophobic acids will retain longer, and their elution can be effectively controlled by changing the ionization state at a different pH. This method is compatible with major detection techniques ( (ELSD, CAD, RI, UV and mass spec). This method can be used for the analysis of basic hydrophobic drugs and their acidic counter-ions in one run. Acidic hydrophobic drugs and related impurities can be analyzed on this mixed-mode HPLC column.

Heritage MA PURCHASE LEARN ABOUT Heritage MA

Conditions of Experiment

Column:	Heritage MA
Separation Modes:	anion-exchange
Column Dimenstions:	4.6x150 mm, 3 um, 100A
Mobile Phase:	60% ACN with 60 mM AmFm pH 3
Detection:	ELSD, 50°C
Sample:	1 mg/ml
Injection:	3 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Inorganic anion, Organic acid
Nature of compounds:	Acidic, Hydrophilic, Polar
Compounds:	Phosphate Ion, Bromate Ion, Bromide ion, Chloride Ion, Iodide ion, Trifluoroacetic acid, Perchlorate ion