Caffeine

HPLC Analysis of Caffeine on Heritage C18 Column According to US Pharmacopeia
Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: NaFm/H2O/MeCN/THF 1.64 g /2L/50 ml/40 ml/ pH 4.5 with acetic acid
Detection: UV 270 nm
Sample: 0.2 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Analytes
Class of compounds: Stimulant, Xanthine
Nature of compounds: Hydrophilic, Neutral
Compounds: Caffeine
HPLC Analysis of Drug Composition on Heritage C18 Column According to US Pharmacopeia
Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 100 mm, 5 um, 100A
Mobile Phase: MeOH/H2O/AcOH 30/67/3
Detection: UV 275 nm
Sample: 0.2 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Analytes
Class of compounds: Aromatic acid, Aromatic compound, Drug, Organic acid, Stimulant, Xanthine
Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: Acetaminophen, Caffeine, Aspirin, Benzoic acid, Salicylic acid
HPLC Method for Analysis of Caffeine and Other Xanthines on Amaze HD Column
Application description

Cytosine is one of four basic compounds that play an important role in building DNA and RNA molecules. Cytidine consist of cytosine and ribose molecules. Cytosine and cytidine molecules are polar weak basis. They have an ability to form hydrogen bonds. Order of elution and selectivity of separation can be changed by changing mobile phase composition. Method is compatible with most common HPLC detection techniques: MS, RI, UV, ELSD, CAD, etc.

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 3.2 x 50 mm
Mobile Phase: MeCN/MeOH (95/5) with 0.1% HCOOH with 0.01%AmFm
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1.0 mL/min
Analytes
Class of compounds: Stimulant, Xanthine
Nature of compounds: Hydrophilic, Neutral, Polar
Compounds: Caffeine, 3-Methylxanthine, 1-Methylxanthine, Xanthine
Selectivity Difference for Polar Compounds in Reversed-Phase and Mixed-Mode Separation on Coresep 100 HPLC Column
Application description

Core-shell mixed-mode columns offer unique selectivity which comes from the presence of two mechanisms - reversed-phase and ion-exchange. The alternative selectivity of mixed-mode columns can be used when order of elution or resolution between compounds needs improvement. Even for neutral compounds a different selectivity can be obtained. A method for common components of headache medications (acetaminophen, caffeine, and benzoate) was developed on our Coresep 100 column. Efficiency and peak shape are comparable to leading brand core-shell reversed-phase columns.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase
Column Dimenstions: 3.2 x 50 mm, 2.7 um, 90A
Mobile Phase: 20% ACN with 0.1% TFA
Detection: UV 250 nm, ELSD
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic acid, Aromatic compound, Drug, Organic acid, Stimulant, Xanthine
Nature of compounds: Acidic, Hydrophilic, Neutral
Compounds: Acetaminophen, Caffeine, Benzoic acid