Caffeine
Column: | Amaze C18 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | 25% ACN with 50 mM ammonium phosphate and 0.1% phosphoric acid |
Detection: | 220 nm |
Sample: | 0.3-0.5 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Aromatic acid, Aromatic compound, Drug, Stimulant, Xanthine |
Nature of compounds: | Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Acetaminophen, Caffeine, Aspirin |
Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C20 columns.
Column: | Amaze AR |
Separation Modes: | reversed-phase, pi-pi |
Column Dimenstions: | 4.6x150 mm, 5 μm, 100A |
Mobile Phase: | 28% ACN with 20 mM AmAc pH 4.5 |
Detection: | UV 255 nm |
Sample: | 1 mg/ml |
Injection: | 3 μL |
Flow rate: | 1 ml/min |
Class of compounds: | Amino acid, Aromatic acid, Aromatic compound, Drug, Isomer, Organic acid, Preservative, Stimulant, Xanthine |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic |
Compounds: | Caffeine, 4-Aminobenzoic acid, Aspirin, 4-Hydroxybenzoic acid, Sorbic acid, Benzoic acid |
Column: | Heritage С18 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 4.6 x 150 mm, 5 um, 100A |
Mobile Phase: | NaFm/H2O/MeCN/THF 1.64 g /2L/50 ml/40 ml/ pH 4.5 with acetic acid |
Detection: | UV 270 nm |
Sample: | 0.2 mg/ml |
Injection: | 5 uL |
Flow rate: | 1 mL/min |
Class of compounds: | Stimulant, Xanthine |
Nature of compounds: | Hydrophilic, Neutral |
Compounds: | Caffeine |
Column: | Heritage С18 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 4.6 x 100 mm, 5 um, 100A |
Mobile Phase: | MeOH/H2O/AcOH 30/67/3 |
Detection: | UV 275 nm |
Sample: | 0.2 mg/ml |
Injection: | 5 uL |
Flow rate: | 1 mL/min |
Class of compounds: | Aromatic acid, Aromatic compound, Drug, Organic acid, Stimulant, Xanthine |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Acetaminophen, Caffeine, Aspirin, Benzoic acid, Salicylic acid |
Cytosine is one of four basic compounds that play an important role in building DNA and RNA molecules. Cytidine consist of cytosine and ribose molecules. Cytosine and cytidine molecules are polar weak basis. They have an ability to form hydrogen bonds. Order of elution and selectivity of separation can be changed by changing mobile phase composition. Method is compatible with most common HPLC detection techniques: MS, RI, UV, ELSD, CAD, etc.
Column: | Amaze HD |
Separation Modes: | HILIC |
Column Dimenstions: | 3.2 x 50 mm |
Mobile Phase: | MeCN/MeOH (95/5) with 0.1% HCOOH with 0.01%AmFm |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1.0 mL/min |
Class of compounds: | Stimulant, Xanthine |
Nature of compounds: | Hydrophilic, Neutral, Polar |
Compounds: | Caffeine, 3-Methylxanthine, 1-Methylxanthine, Xanthine |
Core-shell mixed-mode columns offer unique selectivity which comes from the presence of two mechanisms - reversed-phase and ion-exchange. The alternative selectivity of mixed-mode columns can be used when order of elution or resolution between compounds needs improvement. Even for neutral compounds a different selectivity can be obtained. A method for common components of headache medications (acetaminophen, caffeine, and benzoate) was developed on our Coresep 100 column. Efficiency and peak shape are comparable to leading brand core-shell reversed-phase columns.
Column: | Coresep 100 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 3.2 x 50 mm, 2.7 um, 90A |
Mobile Phase: | 20% ACN with 0.1% TFA |
Detection: | UV 250 nm, ELSD |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Aromatic acid, Aromatic compound, Drug, Organic acid, Stimulant, Xanthine |
Nature of compounds: | Acidic, Hydrophilic, Neutral |
Compounds: | Acetaminophen, Caffeine, Benzoic acid |