CBD

The legalization of medical and recreational marijuana introduced a lot of cannabis infused products. These products need to be analyzed in terms of the content and potency of marijuana related products. Almost every cannabinoid is a hydrophobic neutral compound except some of the acidic cannabinoids like THCA, CBDA, CBGA, etc.).Tetrahydrocannabinol is the main component of marijuana plants. There are only traces of THC in hemp plants. The cannabidiol is the main component of hemp plants and hemp extract, and can go as high as 40% in the extract. Both THCA and CBD are hydrophobic in nature and stay on the column by pure reversed-phase mechanism. In case of cannabis infused edibles, a sample matrix needs to be taken in consideration when developing analytical approaches for the analysis of marijuana related products. We have developed a set of columns and methods for the analysis of marijuana plants, extracts, isolates and edibles. The Daze 47 HPLC column was used for the analysis of THC-CBD infused gummies. Minimal sample preparation was performed and both cannabinoids were analyzed and quantified using UV detection. The Daze 47 column can be used for the analysis of main cannabinoids, as well as contaminants like herbicides in cannabinoids samples. Our method is robust, short and efficient. It can be used for the analysis of tetrahydrocannabinol (THC), tetrahydrocannabinolic acid (THCA), cannabidiol (CBD), cannabidiolic acid (CBDA), cannabinol (CBN), cannabinolic acid (CBNA), cannabigerol (CBG), cannabichromene (CBC), cannabivarin (CBV), cannabicyclol (CBL), tetrahydrocannabivarin (THCV) cannabidivarin (CBDV), cannabichromevarin (CBCV), cannabigerovarin (CBGV), cannabieeeelsoin (CBE) and cannabicitran (CBT)

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Conditions of Experiment

Column:	Daze 47
Separation Modes:
Column Dimenstions:	3.0 x 150 mm, 5 um, 100A
Mobile Phase:	70% ACN with 0.1% H2SO4
Detection:	UV 275 nm
Sample:
Injection:
Flow rate:	1 ml/min

Analytes

Class of compounds:	Aromatic compound, Cannabinoid, Controlled substance, Drug
Nature of compounds:	Hydrophobic, Neutral
Compounds:	CBD, THC

The legalization of medical and recreational marijuana introduced a lot of cannabis infused products. These products need to be analyzed in terms of the content of active cannabinoids, and to determine the potency of marijuana related products. Almost every cannabinoid is a hydrophobic neutral compound except some of the acidic cannabinoids such as THCA, CBDA, CBNA, CBGA, etc. Cannabidiol is one of the minor components of marijuana plants. The cannabidiol is the main component of hemp plants and hemp extract, and can go as high as 40% in the extract. There are only traces of THC in hemp plants. Regulations require that hemp derived product have less than 0.3% of THC/THCA in it in order to be legal across all of the United States. Both THC and CBD are hydrophobic in nature and are retained on the Daze 47 column by pure reversed-phase mechanism. In case of cannabis infused edibles, the sample matrix needs to be taken in consideration when developing analytical approaches for the analysis of marijuana related products. We have developed a set of columns and methods for the analysis of marijuana plants, extracts, isolates and edibles. The Daze 47 HPLC column was used for the analysis of THC-CBD infused gummies. Minimal sample preparation was performed and both cannabinoids were analyzed and quantified using UV detection. The Daze 47 column can be used for the analysis of main cannabinoids as well as contaminants like herbicides in cannabinoids-infused samples. Our method is robust, short and efficient.

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Conditions of Experiment

Column:	Daze 47
Separation Modes:
Column Dimenstions:	3.0 x 150 mm, 5 um, 100A
Mobile Phase:	70% ACN with 0.1% H2SO4
Detection:	UV 275 nm
Sample:
Injection:
Flow rate:	1.0 mL/min

Analytes

Class of compounds:	Aromatic compound, Cannabinoid, Drug
Nature of compounds:	Hydrophobic, Neutral
Compounds:	CBD

With legalization of marijuana and marijuana related products in USA, Canada and other countries there is a need for analysis for potency and contaminants in these products. The method for potency of marijuana requires separation and quantification of main components of marijuana. The main components of marijuana are THC, THCA, CBN, CBD, and CBDA. Tetrahydrocannabinolic acid (THCA) is active component of marijuana and a precursor of tetrahydrocannabinol (THC). It is acidic and hydrophobic in nature. It undergoes decarboxylation to from tetrahydrocannabinol (THC). Tetrahydrocannabinol (THC) is one of dozens of cannabinoids identified in cannabis. It is a psychoactive component. It is Schedule II compound. Cannabidiol (CBD) is one of dozens of cannabinoids. The hemp extract contains up to 40% of CBD and is used to produce this pharmaceutically active ingredient of cannabis. It is used as pain medication and medication to treat epilepsy. CBDA is obtained from CBDA by decarboxylation of cannabidiolic acid (CBDA). Fresh extract of marijuana plants contains 95% THCA and 5% CBDA. Cannabinol (CBN) is non-psychoactive compound found only in traces in cannabis. Unlike other cannabinoids it is not derived from cannabigerol (CBG). CBN is not a controlled substance. We have developed an effective separation of CBD, CBDA, CBN, THC and THCA on Heritage C18 HPLC column. Compounds are separated based on reversed-phase mechanism. The method can be used for analysis of cannabinoids in marijuana, marijuana related products, THC and CBD infused edibles, etc.

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Conditions of Experiment

Column:	Heritage C18
Separation Modes:	reversed-phase
Column Dimenstions:	3.2 x 150 mm, 5 um, 100A
Mobile Phase:	MeCN 70% with 0.1% phosphoric acid
Detection:	UV 275 nm
Sample:	0.3 mg/ml
Injection:	1 uL
Flow rate:	1.2 mL/min

Analytes

Class of compounds:	Aromatic acid, Aromatic compound, Cannabinoid, Controlled substance, Drug
Nature of compounds:	Acidic, Hydrophobic, Neutral
Compounds:	CBDA, CBD, CBN, THC, THCA

Three main cannabinoids (THC, CBD and CBN) were analyzed on a Coresep 100 core-shell mixed-mode column. Tetrahydrocannabinol is a psychoactive component of cannabis.THC is a lipid. The precursor of THC is tetrahydrocannabinolic acid (THCA). Cannabidiol is a major phytocannabinoid. CBD does not have any psychoactive properties, but constitutes up to 40% of plants extracts. Cannabinols are non-psychoactive cannabinoids which occurs in trace amounts in marijuana plants. All three compounds are hydrophobic and neutral, and are separated by reversed-phase mechanism. Method can be used in analysis of active components of various marijuana related products (flowers, buds, edibles, infused products, etc.). HPLC method is reproducible and robust.

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Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase
Column Dimenstions:	3.2 x 100 mm, 2.7 um, 90A
Mobile Phase:	50% ACN with 0.1% of phosphoric acid
Detection:	UV 210 nm
Sample:	0.3 mg/ml
Injection:	3 uL
Flow rate:	1.5 ml/min

Analytes

Class of compounds:	Aromatic compound, Cannabinoid, Controlled substance, Drug
Nature of compounds:	Hydrophobic, Neutral
Compounds:	CBD, CBN, THC