HPLC Separation of Critical Pairs of Cannabinoids (CBD/CBG, d8-THC and d9-THC) on Amaze SPF Column
Conditions of Experiment
Column: Amaze SPF
Separation Modes: reversed-phase, pi-pi, dipol-dipol
Column Dimenstions: 4.6x250 mm, 5 um, 100A
Mobile Phase: 60% MeH with 10 mM AmAc pH 4
Detection: 275 nm
Sample: 0.05-0.3 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic compound, Cannabinoid, Drug
Nature of compounds: Hydrophobic, Neutral
Compounds: CBD, Iso-Tetrahydrocannabinol, CBG, Delta-8-Tetrahydrocannabinol, Delta-9-Tetrahydrocannabinol, d9-THCA
HPLC Analysis of Cannabis Infused Syrup
Conditions of Experiment
Column: Daze 47
Separation Modes:
Column Dimenstions: 3.0 x 150 mm, 5 um, 100A
Mobile Phase: 70% ACN with 0.1% H2SO4
Detection: UV 275 nm
Flow rate: 1.0 mL/min
Class of compounds: Aromatic acid, Aromatic compound, Cannabinoid, Controlled substance, Drug
Nature of compounds: Acidic, Hydrophobic, Neutral
Compounds: CBG, CBD, CBN, THC, THCA