HPLC Analysis of Five Main Cannabinoids on Heritage C18 Column
Application description
With legalization of marijuana and marijuana related products in USA, Canada and other countries there is a need for analysis for potency and contaminants in these products. The method for potency of marijuana requires separation and quantification of main components of marijuana. The main components of marijuana are THC, THCA, CBN, CBD, and CBDA. Tetrahydrocannabinolic acid (THCA) is active component of marijuana and a precursor of tetrahydrocannabinol (THC). It is acidic and hydrophobic in nature. It undergoes decarboxylation to from tetrahydrocannabinol (THC). Tetrahydrocannabinol (THC) is one of dozens of cannabinoids identified in cannabis. It is a psychoactive component. It is Schedule II compound. Cannabidiol (CBD) is one of dozens of cannabinoids. The hemp extract contains up to 40% of CBD and is used to produce this pharmaceutically active ingredient of cannabis. It is used as pain medication and medication to treat epilepsy. CBDA is obtained from CBDA by decarboxylation of cannabidiolic acid (CBDA). Fresh extract of marijuana plants contains 95% THCA and 5% CBDA. Cannabinol (CBN) is non-psychoactive compound found only in traces in cannabis. Unlike other cannabinoids it is not derived from cannabigerol (CBG). CBN is not a controlled substance. We have developed an effective separation of CBD, CBDA, CBN, THC and THCA on Heritage C18 HPLC column. Compounds are separated based on reversed-phase mechanism. The method can be used for analysis of cannabinoids in marijuana, marijuana related products, THC and CBD infused edibles, etc.
Conditions of Experiment
| Column: |
Heritage C18 |
| Separation Modes: |
reversed-phase |
| Column Dimenstions: |
3.2 x 150 mm, 5 um, 100A |
| Mobile Phase: |
MeCN 70% with 0.1% phosphoric acid |
| Detection: |
UV 275 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
1.2 mL/min |
Analytes
| Class of compounds: |
Aromatic acid, Aromatic compound, Cannabinoid, Controlled substance, Drug |
| Nature of compounds: |
Acidic, Hydrophobic, Neutral |
| Compounds: |
CBDA, CBD, CBN, THC, THCA |
HPLC Analysis of Three Cannabinoids on Cores-Shell Mixed-Mode Coresep 100 Column
Application description
Three main cannabinoids (THC, CBD and CBN) were analyzed on a Coresep 100 core-shell mixed-mode column. Tetrahydrocannabinol is a psychoactive component of cannabis.THC is a lipid. The precursor of THC is tetrahydrocannabinolic acid (THCA). Cannabidiol is a major phytocannabinoid. CBD does not have any psychoactive properties, but constitutes up to 40% of plants extracts. Cannabinols are non-psychoactive cannabinoids which occurs in trace amounts in marijuana plants. All three compounds are hydrophobic and neutral, and are separated by reversed-phase mechanism. Method can be used in analysis of active components of various marijuana related products (flowers, buds, edibles, infused products, etc.). HPLC method is reproducible and robust.
Conditions of Experiment
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase |
| Column Dimenstions: |
3.2 x 100 mm, 2.7 um, 90A |
| Mobile Phase: |
50% ACN with 0.1% of phosphoric acid |
| Detection: |
UV 210 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
1.5 ml/min |
Analytes
| Class of compounds: |
Aromatic compound, Cannabinoid, Controlled substance, Drug |
| Nature of compounds: |
Hydrophobic, Neutral |
| Compounds: |
CBD, CBN, THC |
