Chloramphenicol

HPLC Analysis of Drug Chloramphenicol on Heritage C18 Column According to US Pharmacopeia
Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 100 mm, 5 um, 100A
Mobile Phase: MeOH/H2O/AcOH 45/55/0.1
Detection: UV 270 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Analytes
Class of compounds: Antibiotic, Drug
Nature of compounds: Hydrophobic, Neutral
Compounds: Chloramphenicol
HPLC Analysis of Antibiotic Chloramphenicol on Coresep 100 Mixed-Mode Column
Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM in 7 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Analytes
Class of compounds: Antibiotic, Drug
Nature of compounds: Hydrophobic, Neutral
Compounds: Chloramphenicol