Chloride Ion
Column: | Amaze TH |
Separation Modes: | HILIC/cation-exchnage, anion-exchange |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | ACN/water/ammonium formate pH 3 |
Detection: | ELSD |
Sample: | 0.4-0.6 |
Injection: | 3 uL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Amino acid, Inorganic anion, Supplement |
Nature of compounds: | Acidic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Chloride Ion, Taurine, Threonine, Serine, Arginine |
Column: | Amaze TCH |
Separation Modes: | HILIC/cation-exchnage, anion-exchange |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | ACN/water/ammonium formate pH 3 |
Detection: | ELSD |
Sample: | 0.4-0.6 |
Injection: | 3 uL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Amino acid, Inorganic anion, Supplement |
Nature of compounds: | Acidic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Chloride Ion, Taurine, Threonine, Serine, Arginine |
Column: | Amaze TH |
Separation Modes: | HILIC, anion-exchange |
Column Dimenstions: | 4.6x150 mm, 5 um, 100A |
Mobile Phase: | 80% ACN with 10 mM AmFm pH 3 |
Detection: | ELSD |
Sample: | 0.3-0.6 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Herbicide, Inorganic anion, Organic acid, Quaternary amine |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Chloride Ion, Ethephon, Mepiquat |
Column: | Amaze HD |
Separation Modes: | HILIC, cation-exchange, anion-exclusion |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | 75% ACN with 50 mM AmAc pH 5 |
Detection: | ELSD |
Sample: | 0.2-0.5 mg/ml |
Injection: | 2 uL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Amino acid, Inorganic anion, Sugar, Supplement |
Nature of compounds: | Acidic, Hydrophilic, Neutral, Polar, Zwitterionic |
Compounds: | Chloride Ion, Sucrose, Histidine, Arginine |
Column: | Amaze TCH |
Separation Modes: | HILIC, cation-exchange, anion-exchange |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | from 80% ACN with 5 mM ammonium formate pH 3.5 to 40% ACN with 30 mM ammonium formate pH 3.5 in 10 min |
Detection: | Corona CAD |
Sample: | 0.2-0.5 mg/ml |
Injection: | 2 ul |
Flow rate: | 1 ml/min |
Class of compounds: | Amines, Herbicide, Inorganic anion, Organic acid, Quaternary amine |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Chlormequat, Mepiquat, Paraquat, Diquat, Chloride Ion, Ethephon |
Column: | Amaze TCH |
Separation Modes: | HILIC, cation-exchange, anion-exchange |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | 73% ACN with 20 mM AmFm pH 2.7 |
Detection: | Corona CAD |
Sample: | 0.5 mg/ml |
Injection: | 2 ul |
Flow rate: | 1 ml/min |
Class of compounds: | Inorganic anion, Inorganic cation, Organic acid |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Iodide ion, Potassium ion, Sodium ion, Lithium ion, Bromide ion, Chloride Ion |
Column: | Amaze TH |
Separation Modes: | HILIC cation-exchange, anion-exchange |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | 40% ACN with gradient of AmFm pH 3 from 30 mM to 120 mM in 7.5 min, hold 8 min |
Detection: | ELSD |
Sample: | 0.5-1 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Inorganic acid, Inorganic anion, Inorganic cation, Organic acid |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Phosphate Ion, Chloride Ion, Sodium ion, Iodide ion, Sulfate ion |
Column: | Amaze HD |
Separation Modes: | HILIC, cation-exchange, anion-exclusion |
Column Dimenstions: | 3.0x100 mm, 3 μm, 100A |
Mobile Phase: | 82% ACN with 36 mM AmFm pH 3 |
Detection: | ELSD |
Sample: | 1 mg/ml |
Injection: | 2 μL |
Flow rate: | 1 ml/min |
Class of compounds: | Amines, Amino acid, Drug, Inorganic anion, Sugar, Supplement, Vitamin |
Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic |
Compounds: | Chloride Ion, Choline, Betaine, L-Carnitine, Inositol |
Column: | Amaze TH, Amaze HD |
Separation Modes: | HILIC, cation-exchange, anion-exchange, anion-exclusion |
Column Dimenstions: | 3.0x100 mm, 3 μm, 100A |
Mobile Phase: | 85% ACN with 30 mM AmFm pH 3 |
Detection: | Corona CAD |
Sample: | 1 mg/ml |
Injection: | 2 μL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Amines, Aromatic base, Inorganic anion, Inorganic cation, Organic acid |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Nitrate ion, Chloride Ion, Guanidine, Sodium ion |
Column: | Amaze HD |
Separation Modes: | HILIC, cation-exchange and anion-exclusion |
Column Dimenstions: | 3.0x100 mm, 3 μm, 100A |
Mobile Phase: | 72% ACN with 56 mM AmFm pH 3 |
Detection: | Corona CAD |
Sample: | 1 mg/ml |
Injection: | 2 μL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Amino acid, Aromatic acid, Catecholamine, Drug, Inorganic anion, Neurotransmitter, Supplement |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Chloride Ion, Citrulline, 4-Aminobuyric acid (GABA), Ornithine, Dopamine, Arginine |
Column: | Amaze SC |
Separation Modes: | reversed-phase and cation-exchange |
Column Dimenstions: | 3.0x100 mm, 5μm, 100A |
Mobile Phase: | 10% ACN with 20 mM AmFm pH 3 |
Detection: | Corona CAD |
Sample: | 0.5 mg/ml |
Injection: | 2 μL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Amino acid, Aromatic acid, Catecholamine, Drug, Inorganic anion, Neurotransmitter, Supplement |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Chloride Ion, Citrulline, 4-Aminobuyric acid (GABA), Ornithine, Dopamine, Arginine |
Column: | Amaze HD |
Separation Modes: | HILIC, cation-exchange, anion-exclusion |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | 75% ACN with 50 mM AmAc pH 5 |
Detection: | ELSD, 40°C |
Sample: | 2 mg/ml |
Injection: | 3 uL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Amino acid, Inorganic anion, Sugar, Supplement |
Nature of compounds: | Acidic, Hydrophilic, Neutral, Polar, Zwitterionic |
Compounds: | Chloride Ion, Sucrose, Histidine, Arginine |
Organic and inorganic acids are part of many formulations including pharmaceutical and nutraceuticals. These compounds are very often very polar and acidic, and either require expensive ion-chromatography or the use of ion-pairing reagents. Ion-pairing reagents are not compatible with mass spectrometry. Mixed-mode columns allow the utilization of the ion-pairing reagent attached to the surface of silica gel to retain these anionic compounds. The retention is provided by weak reverse-phase and by a medium or strong anion-exchange mechanism. The retention time is controlled by the amount of ACN, buffer pH and buffer concentration. Stronger and more hydrophobic acids will retain longer, and their elution can be effectively controlled by changing the ionization state at a different pH. This method is compatible with major detection techniques ( (ELSD, CAD, RI, UV and mass spec). This method can be used for the analysis of basic hydrophobic drugs and their acidic counter-ions in one run. Acidic hydrophobic drugs and related impurities can be analyzed on this mixed-mode HPLC column.
Column: | Heritage MA |
Separation Modes: | anion-exchange |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | 60% ACN with 60 mM AmFm pH 3 |
Detection: | ELSD, 50°C |
Sample: | 1 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Inorganic anion, Organic acid |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Phosphate Ion, Bromate Ion, Bromide ion, Chloride Ion, Iodide ion, Trifluoroacetic acid, Perchlorate ion |