Chloride Ion

HILIC HPLC Analysis of Dietary Supplement Composition Containing Choline, Carnitine, Betaine and Inositol on Amaze HD Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 μm, 100A
Mobile Phase: 82% ACN with 36 mM AmFm pH 3
Detection: ELSD
Sample: 1 mg/ml
Injection: 2 μL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amines, Amino acid, Drug, Inorganic anion, Sugar, Supplement, Vitamin
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic
Compounds: Chloride Ion, Choline, Betaine, L-Carnitine, Inositol
HPLC Analysis of Guanidine and Counterions in in HILIC Ion-Exchange and HIIC Ion-Exclusion Modes
Conditions of Experiment
Column: Amaze TH, Amaze HD
Separation Modes: HILIC, cation-exchange, anion-exchange, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 μm, 100A
Mobile Phase: 85% ACN with 30 mM AmFm pH 3
Detection: Corona CAD
Sample: 1 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amines, Aromatic base, Inorganic anion, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Nitrate ion, Chloride Ion, Guanidine, Sodium ion
HPLC Analysis of Dopamine and 5 Amino Acids in HILIC and Cation-Exchange Modes on Amaze HD Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange and anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 μm, 100A
Mobile Phase: 72% ACN with 56 mM AmFm pH 3
Detection: Corona CAD
Sample: 1 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amino acid, Aromatic acid, Catecholamine, Drug, Inorganic anion, Neurotransmitter, Supplement
Nature of compounds: Acidic, Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Chloride Ion, Citrulline, 4-Aminobuyric acid (GABA), Ornithine, Dopamine, Arginine
HPLC Analysis of Dopamine and 5 Amino Acids in Reversed-Phase and Cation-Exchange Modes on Amaze SC Column
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.0x100 mm, 5μm, 100A
Mobile Phase: 10% ACN with 20 mM AmFm pH 3
Detection: Corona CAD
Sample: 0.5 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amino acid, Aromatic acid, Catecholamine, Drug, Inorganic anion, Neurotransmitter, Supplement
Nature of compounds: Acidic, Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Chloride Ion, Citrulline, 4-Aminobuyric acid (GABA), Ornithine, Dopamine, Arginine
HPLC Analysis of Sucrose, Histidine and Arginine on Amaze HD Mixed-Mode Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 75% ACN with 50 mM AmAc pH 5
Detection: ELSD, 40°C
Sample: 2 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amino acid, Inorganic anion, Sugar, Supplement
Nature of compounds: Acidic, Hydrophilic, Neutral, Polar, Zwitterionic
Compounds: Chloride Ion, Sucrose, Histidine, Arginine
HPLC Analysis of Seven Anions on Heritage MA Mixed-Mode Column
Application description

Organic and inorganic acids are part of many formulations including pharmaceutical and nutraceuticals. These compounds are very often very polar and acidic, and either require expensive ion-chromatography or the use of ion-pairing reagents. Ion-pairing reagents are not compatible with mass spectrometry. Mixed-mode columns allow the utilization of the ion-pairing reagent attached to the surface of silica gel to retain these anionic compounds. The retention is provided by weak reverse-phase and by a medium or strong anion-exchange mechanism. The retention time is controlled by the amount of ACN, buffer pH and buffer concentration. Stronger and more hydrophobic acids will retain longer, and their elution can be effectively controlled by changing the ionization state at a different pH. This method is compatible with major detection techniques ( (ELSD, CAD, RI, UV and mass spec). This method can be used for the analysis of basic hydrophobic drugs and their acidic counter-ions in one run. Acidic hydrophobic drugs and related impurities can be analyzed on this mixed-mode HPLC column.

Conditions of Experiment
Column: Heritage MA
Separation Modes: anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 60% ACN with 60 mM AmFm pH 3
Detection: ELSD, 50°C
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic anion, Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Phosphate Ion, Bromate Ion, Bromide ion, Chloride Ion, Iodide ion, Trifluoroacetic acid, Perchlorate ion