Application description
Ciprofloxacin is an antibiotic drug used to treat bacterial infections. It does not treat viral infections like common cold. Ciprofloxacin is the most widely used of the second-generation quinolones. The molecule is hydrophobic and zwitterionic in nature. Carboxylic acid ionization on the molecule can be suppressed by using low pH mobile phase (below 3). Ciprofloxacin and related impurities were separated on Coresep SB mixed-mode column in reversed-phase cation-exclusion modes. Method is fast, robust, reproducible, and can be used for analysis of ciprofloxacin in various matrices (reaction mixtures, formulations, biofluids). Various mobile phases can be used with Coresep SB column to accommodate different detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.
Conditions of Experiment
| Column: |
Coresep SB |
| Separation Modes: |
reversed-phase and cation-exchange |
| Column Dimenstions: |
3.0 x 100 mm |
| Mobile Phase: |
5% ACN with 0.2% of H3PO4 |
| Detection: |
UV 215 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.6 mL/min |
Analytes
| Class of compounds: |
Antibiotic, Aromatic acid, Aromatic base, Drug |
| Nature of compounds: |
Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic |
| Compounds: |
Ciprofloxacin, Related Impurities |
