Application description
This UV HPLC method is tailored for the analysis of copper and iron ions utilizing an Amaze HA mixed-mode column. Ethylenediaminetetraacetic acid (EDTA) is employed as a visualization agent, forming colored complexes with iron and copper ions, thereby enabling their monitoring via UV detection.
Copper and iron ions are essential trace elements vital for various biological processes. While copper ions play a crucial role in enzyme function, iron ions are integral components of hemoglobin and enzymes involved in oxygen transport and metabolism.
Both copper and iron ions exhibit variable coordination chemistry, allowing them to form complexes with chelating agents such as EDTA. These complexes contribute to the detection of copper and iron ions in solution through UV-visible spectroscopy.
Ethylenediaminetetraacetic acid (EDTA) is a polydentate chelating agent widely used in analytical chemistry for its ability to form stable complexes with metal ions. EDTA forms a colored complex with both copper and iron ions, allowing for their sensitive detection via UV absorption.
The Amaze HA mixed-mode column is selected for its versatility in handling polar and charged analytes. In this method, the column facilitates separation based on differences in hydrophobicity and charge interactions, ensuring efficient retention and resolution of copper and iron ions.
By utilizing this UV HPLC method with EDTA as a visualization agent, sensitive and selective detection of copper and iron ions can be achieved, facilitating their analysis in various environmental, pharmaceutical, and biological samples.
Conditions of Experiment
| Column: |
Amaze HA |
| Separation Modes: |
Anion-exchange |
| Column Dimenstions: |
3x100 mm, 3 um, 100 A |
| Mobile Phase: |
ACN/Water/ammonium formate with EDTA |
| Detection: |
UV 295 nm |
| Sample: |
0.1-0.1 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
0.6 ml/min |
Analytes
| Class of compounds: |
Contaminant, Inorganic anion, Inorganic cation |
| Nature of compounds: |
Basic, Hydrophilic |
| Compounds: |
Copper ion, Iron ion |
