Cysteine

HPLC Analysis of 20 Amino Acids on Amaze SC Mixed-Mode Column in RP and Cation-exchange Modes
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase cation-exchange
Column Dimenstions: 4.6x250 mm, 5 μm, 100A
Mobile Phase: A: 2% ACN with 0.1% TFA B: 25% ACN with 0.3% TFA from 100% A to 100% B in 20 min, 8 min hold
Detection: Corona CAD
Sample: 0.2 mg/ml
Injection: 5 μL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amino acid, Neurotransmitter, Supplement
Nature of compounds: Acidic, Hydrophilic, Polar, Zwitterionic
Compounds: Aspartic acid, Serine, Asparagine, Cysteine, Glutamic acid, Threonine, Glutamine, Glycine, Alanine, Proline, Valine, Methionine, Tyrosine, Isoleucine, Leucine, Phenylalanine, Lysine, Histidine, Tryptophan, Arginine
UV HPLC Analysis of Cysteine, Carbocystein and Cystine on Coresep 100 Mixed-Mode Column
Conditions of Experiment
Column: Coresep 100
Separation Modes: reveresed-phase cation-exchange
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: ACN/water/phosphoric acid gradinet
Detection: UV 230 nm
Sample: 0.5 mg/ml
Injection:
Flow rate: 1 ml/min
Analytes
Class of compounds: Amino acid, Supplement
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic
Compounds: Cysteine, Carbocysteine, Cystine
HPLC Analysis of Cysteine, Cystine and Carbocysteine on Amaze HD Mixed-Mode Column in Isocratic Mode
Conditions of Experiment
Column: Anaze HD
Separation Modes: HILIC, cation-exchange, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 75% ACN with 50 mM AmAc pH 4
Detection: ELSD, 40*C
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amino acid, Supplement
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic
Compounds: Cysteine, Carbocysteine, Cystine
HPLC Analysis of Cysteine, Cystine and Carbocysteine on Amaze HD Mixed-Mode Column in Gradient Mode
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: ACN/water/AmAc gradient
Detection: ELSD, 40*C
Sample: 2 mg/ml
Injection: 2 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amino acid, Supplement
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic
Compounds: Cysteine, Carbocysteine, Cystine
HPLC Analysis of 18 Amino Acids on Amaze HD Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x100 mm, 5 um, 100A
Mobile Phase: 80% ACN with 20 mM AmFm pH 3
Detection: ELSD, 45°C
Sample: 0.5-0.8 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amino acid, Catecholamine, Drug, Neurotransmitter, Supplement
Nature of compounds: Acidic, Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Tryptophan, Phenylalanine, Leucine, Methionine, Tyrosine, Isoleucine, DOPA, Valine, Cysteine, Proline, Alanine, Threonine, Glutamic acid, Glycine, Glutamine, Asparagine, Serine, Aspartic acid