Cysteine

UV HPLC Analysis of Cysteine, Carbocystein and Cystine on Coresep 100 Mixed-Mode Column
Conditions of Experiment
Column: Coresep 100
Separation Modes: reveresed-phase cation-exchange
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: ACN/water/phosphoric acid gradinet
Detection: UV 230 nm
Sample: 0.5 mg/ml
Injection:
Flow rate: 1 ml/min
Analytes
Class of compounds: Amino acid, Supplement
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic
Compounds: Cysteine, Carbocysteine, Cystine
HPLC Analysis of Cysteine, Cystine and Carbocysteine on Amaze HD Mixed-Mode Column in Isocratic Mode
Conditions of Experiment
Column: Anaze HD
Separation Modes: HILIC, cation-exchange, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 75% ACN with 50 mM AmAc pH 4
Detection: ELSD, 40*C
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amino acid, Supplement
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic
Compounds: Cysteine, Carbocysteine, Cystine
HPLC Analysis of Cysteine, Cystine and Carbocysteine on Amaze HD Mixed-Mode Column in Gradient Mode
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: ACN/water/AmAc gradient
Detection: ELSD, 40*C
Sample: 2 mg/ml
Injection: 2 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amino acid, Supplement
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic
Compounds: Cysteine, Carbocysteine, Cystine
HPLC Analysis of 18 Amino Acids on Amaze HD Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x100 mm, 5 um, 100A
Mobile Phase: 80% ACN with 20 mM AmFm pH 3
Detection: ELSD, 45°C
Sample: 0.5-0.8 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amino acid, Catecholamine, Drug, Neurotransmitter, Supplement
Nature of compounds: Acidic, Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Tryptophan, Phenylalanine, Leucine, Methionine, Tyrosine, Isoleucine, DOPA, Valine, Cysteine, Proline, Alanine, Threonine, Glutamic acid, Glycine, Glutamine, Asparagine, Serine, Aspartic acid