Cytidine

Cytidine C9H13N3O5

Cytidine

Applications

HPLC Method for Analysis of Cytosine and Cytidine on Amaze HD Column

HPLC Method for Analysis of Cytosine and Cytidine on Amaze HD Column chromatogram
Conditions of Experiment
Column:
Amaze HD
Separation Modes:
HILIC
Column Dimenstions: 3.2 x 50 mm
Mobile Phase: MeCN/MeOH (95/5) with 0.5% HCOOH with 0.05%AmFm
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1.2 mL/min
Analytes
1. Cytidine
2. Cytosine

Class of compounds: Nucleobase, Nucleoside
Nature of compounds: Basic, Hydrophilic, Polar

Application description

Separation of Nine Nucleotides with Core-Shell Mixed-Mode Column

Separation of Nine Nucleotides with Core-Shell Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Coresep SB
Separation Modes:
reversed-phase, anion-exchange
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: 5% ACN, AmAc buffer (pH 4.5) gradient from 20 mmol to 300 mmol in 45 minutes
Detection: UV 270 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1.5 ml/min
Analytes
1. Cytidine
2. Guanosine monophosphate
3. Guanosine diphosphate
4. Guanosine triphosphate
5. Adenosine

Class of compounds: Amino acid, Aromatic acid, Aromatic base, Nucleoside
Nature of compounds: Acidic, Basic, Hydrophilic, Polar

Application description

Core-shell columns are able to handle higher flow-rates, while maintaining high efficiency and low back-pressure. Coresep SB was used to achieve separation of nine nucleotides. The strong embedded basic ion-pairing groups make it possible to separate compounds that don’t retain on reverse-phase columns.

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