Cytidine Cytidine Cytidine Select Applications HPLC Separation of Nucleobases and Nucleosides on Amaze TCH HILIC Mixed-Mode Column HPLC Method for Analysis of Cytosine and Cytidine on Amaze HD Column Separation of Nine Nucleotides with Core-Shell Mixed-Mode Column HPLC Separation of Nucleobases and Nucleosides on Amaze TCH HILIC Mixed-Mode Column Amaze TCH PURCHASE LEARN ABOUT Amaze TCH Conditions of Experiment Column: Amaze TCH Separation Modes: HILIC, cation-exchange Column Dimenstions: 4.6x150 mm, 3 um, 100A Mobile Phase: ACN from 92% to 70% in 15 min, 10 mM AmAc pH 5 Detection: UV 255 nm Sample: 0.2-0.5 mg/ml Injection: 2 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic base, Nucleobase, Nucleoside Nature of compounds: Basic, Hydrophilic, Neutral, Polar Compounds: Thymine, Uracil, Thymidine, Uridine, Adenine, Adenosine, Cytosine, Inosine, Guanine, Cytidine, Guanosine HPLC Method for Analysis of Cytosine and Cytidine on Amaze HD Column Amaze HD PURCHASE LEARN ABOUT Amaze HD Conditions of Experiment Column: Amaze HD Separation Modes: HILIC Column Dimenstions: 3.2 x 50 mm Mobile Phase: MeCN/MeOH (95/5) with 0.5% HCOOH with 0.05%AmFm Detection: UV 275 nm Sample: 0.3 mg/ml Injection: 1 uL Flow rate: 1.2 mL/min Analytes Class of compounds: Nucleobase, Nucleoside Nature of compounds: Basic, Hydrophilic, Polar Compounds: Cytidine, Cytosine Separation of Nine Nucleotides with Core-Shell Mixed-Mode Column Application description Core-shell columns are able to handle higher flow-rates, while maintaining high efficiency and low back-pressure. Coresep SB was used to achieve separation of nine nucleotides. The strong embedded basic ion-pairing groups make it possible to separate compounds that don’t retain on reverse-phase columns. Coresep SB PURCHASE LEARN ABOUT Coresep SB Conditions of Experiment Column: Coresep SB Separation Modes: reversed-phase, anion-exchange Column Dimenstions: 4.6 x 150 mm, 5 um, 100A Mobile Phase: 5% ACN, AmAc buffer (pH 4.5) gradient from 20 mmol to 300 mmol in 45 minutes Detection: UV 270 nm Sample: 0.3 mg/ml Injection: 1 uL Flow rate: 1.5 ml/min Analytes Class of compounds: Amino acid, Aromatic acid, Aromatic base, Nucleoside Nature of compounds: Acidic, Basic, Hydrophilic, Polar Compounds: Cytidine, Guanosine monophosphate, Guanosine diphosphate, Guanosine triphosphate, Adenosine
