HPLC Method for Analysis of Cytosine and Cytidine on Amaze HD Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 3.2 x 50 mm
Mobile Phase: MeCN/MeOH (95/5) with 0.5% HCOOH with 0.05%AmFm
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1.2 mL/min
Class of compounds: Nucleobase, Nucleoside
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Cytidine, Cytosine
Separation of Nine Nucleotides with Core-Shell Mixed-Mode Column
Application description

Core-shell columns are able to handle higher flow-rates, while maintaining high efficiency and low back-pressure. Coresep SB was used to achieve separation of nine nucleotides. The strong embedded basic ion-pairing groups make it possible to separate compounds that don’t retain on reverse-phase columns.

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase, anion-exchange
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: 5% ACN, AmAc buffer (pH 4.5) gradient from 20 mmol to 300 mmol in 45 minutes
Detection: UV 270 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1.5 ml/min
Class of compounds: Amino acid, Aromatic acid, Aromatic base, Nucleoside
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Cytidine, Guanosine monophosphate, Guanosine diphosphate, Guanosine triphosphate, Adenosine