Delta-9-Tetrahydrocannabinol

HPLC Separation of Critical Pairs of Cannabinoids (CBD/CBG, d8-THC and d9-THC) on Amaze SPF Column
Conditions of Experiment
Column: Amaze SPF
Separation Modes: reversed-phase, pi-pi, dipol-dipol
Column Dimenstions: 4.6x250 mm, 5 um, 100A
Mobile Phase: 60% MeH with 10 mM AmAc pH 4
Detection: 275 nm
Sample: 0.05-0.3 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic compound, Cannabinoid, Drug
Nature of compounds: Hydrophobic, Neutral
Compounds: CBD, Iso-Tetrahydrocannabinol, CBG, Delta-8-Tetrahydrocannabinol, Delta-9-Tetrahydrocannabinol, d9-THCA
HPLC Separation of Isomers of Tetrahydrocannabinol on Coresep SPF Column
Conditions of Experiment
Column: Coresep SPF
Separation Modes: reveresed-phase, pi-pi
Column Dimenstions: 4.6x150 mm, 2.7 um, 90A
Mobile Phase: 60% MeOH with 10 mM AmFm pH 3
Detection: 275 nm
Sample: 0.2-0.5 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic compound, Drug
Nature of compounds: Hydrophobic
Compounds: Iso-Tetrahydrocannabinol, Delta-8-Tetrahydrocannabinol, Delta-9-Tetrahydrocannabinol