HPLC Analysis of Plant Growth Regulator Diclobutrazol and Related Impurities on Coresep 100 Mixed-Mode Column
|Conditions of Experiment|
2. Related Impurities
Class of compounds: Aromatic acid, Aromatic base, Drug, Fungicide, Herbicide
|Nature of compounds: Basic, Hydrophilic, Hydrophobic, Neutral|
Diclobutrazol is a racemic mixture of two chemical compounds from the triazole group, which acts as a fungicide. Itis normally applied to the soil to be taken up by the roots and transported via the xylem to the upper parts of the plant. Seeds can be soaked with diclobutrazol to reduce seedling growth. Diclobutrazol is hydrophobic and slightly basic. It is retained on Coresep 100 column by combining a strong reversed-phase mechanism with a weak cation-exchange mechanism. Retention time is controlled by amount of ACN and to a lesser degree by buffer concentration and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of diclobutrazol in various matrices (crops, soil, waste water, etc.). Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.