Application description
Malonic acids are dicarboxylic acids which are polar and thus poorly retained in reversed-phase. An Ultra-fast method for separation of homologs of malonic acids on core-shell mixed-mode columns was developed. Column and method can be used for fast isolation of hydrophilic acids in reversed-phase and anion-exchange modes. Base-line separation with a perfect shape is achieved for all compounds. No ion-pairing reagent is involved.
Conditions of Experiment
| Column: |
Coresep SB |
| Separation Modes: |
reversed-phase and anion-exchange |
| Column Dimenstions: |
3.2 x 100 mm, 2.7 um, 90A |
| Mobile Phase: |
ACN with sulfuric acid |
| Detection: |
UV 215 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
1 ml/min |
Analytes
| Class of compounds: |
Organic acid |
| Nature of compounds: |
Acidic, Hydrophilic, Polar |
| Compounds: |
Malonic acid, Methylmalonic acid, Dimethylmalonic acid |
