Dimethylmalonic acid
Ultra-Fast HPLC Separation of Malonic Acids on Coresep SB Column in Reversed-Phase and Anion-Exchange Modes
Application description
Malonic acids are dicarboxylic acids which are polar and thus poorly retained in reversed-phase. An Ultra-fast method for separation of homologs of malonic acids on core-shell mixed-mode columns was developed. Column and method can be used for fast isolation of hydrophilic acids in reversed-phase and anion-exchange modes. Base-line separation with a perfect shape is achieved for all compounds. No ion-pairing reagent is involved.
Conditions of Experiment
Column: | Coresep SB |
Separation Modes: | reversed-phase and anion-exchange |
Column Dimenstions: | 3.2 x 100 mm, 2.7 um, 90A |
Mobile Phase: | ACN with sulfuric acid |
Detection: | UV 215 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Organic acid |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Malonic acid, Methylmalonic acid, Dimethylmalonic acid |