HPLC Separation of Basic Quaternary and Acidic Herbicides in HILIC, Cation- and Anion-Exchange Modes on Amaze TCH
Conditions of Experiment
Column: Amaze TCH
Separation Modes: HILIC, cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: from 80% ACN with 5 mM ammonium formate pH 3.5
to 40% ACN with 30 mM ammonium formate pH 3.5 in 10 min
Detection: Corona CAD
Sample: 0.2-0.5 mg/ml
Injection: 2 ul
Flow rate: 1 ml/min
Class of compounds: Amines, Herbicide, Inorganic anion, Organic acid, Quaternary amine
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Chlormequat, Mepiquat, Paraquat, Diquat, Chloride Ion, Ethephon