DOPA DOPA DOPA Select Applications HPLC Analysis of 18 Amino Acids on Amaze HD Column Separation of Neurotransmitters on Coresep 100 Column in Reversed-Phase and Cation-Exchange Modes HPLC Analysis of Underivatized Amino Acids on Coresep S Column in HILIC and Cation-Exchange Modes HPLC Analysis of 18 Amino Acids on Amaze HD Column Amaze HD PURCHASE LEARN ABOUT Amaze HD Conditions of Experiment Column: Amaze HD Separation Modes: HILIC, cation-exchange Column Dimenstions: 3.0x100 mm, 5 um, 100A Mobile Phase: 80% ACN with 20 mM AmFm pH 3 Detection: ELSD, 45°C Sample: 0.5-0.8 mg/ml Injection: 3 uL Flow rate: 0.6 ml/min Analytes Class of compounds: Amino acid, Catecholamine, Drug, Neurotransmitter, Supplement Nature of compounds: Acidic, Basic, Hydrophilic, Polar, Zwitterionic Compounds: Tryptophan, Phenylalanine, Leucine, Methionine, Tyrosine, Isoleucine, DOPA, Valine, Cysteine, Proline, Alanine, Threonine, Glutamic acid, Glycine, Glutamine, Asparagine, Serine, Aspartic acid Separation of Neurotransmitters on Coresep 100 Column in Reversed-Phase and Cation-Exchange Modes Application description Catecholamines (neurotransmitters) are derivatives of the amino acid tyrosine. DOPA, tyrosine, phenylalanine, norepinephrine, epinephrine, and dopamine. Fast and efficient method with base-line separation was achieved on Coresep 100 column. All compounds are separated by combination of reversed-phase and cation-exchange mechanisms. Peak order and retention time can be changed by switching from TFA to ammonium formate in the mobile phase, by adjusting mobile phase composition and by changing pH. The method is fully compatible with mass spectroscopy and can be used for fast analysis of neurotransmitters in biofluids. regular. Method can be used a replacement for analysis of neurotransmitters by UPLC with ion-pairing reagents. Coresep 100 PURCHASE LEARN ABOUT Coresep 100 Conditions of Experiment Column: Coresep 100 Separation Modes: reversed-phase and cation-exchange Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A Mobile Phase: ACN gradient from 5% to 10% in 5 min, buffer gradient - Ammonium formate pH 2.9 from 5 to 25 mM in 5 min Detection: UV 270 nm, ELSD Sample: 0.3 mg/ml Injection: 1 uL Flow rate: 1 ml/min Analytes Class of compounds: Amino acid, Aromatic base, Catecholamine, Drug, Neurotransmitter, Supplement Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic Compounds: Dopamine, Tyrosine, Phenylalanine, Norepinephrine, Epinephrine, DOPA HPLC Analysis of Underivatized Amino Acids on Coresep S Column in HILIC and Cation-Exchange Modes Application description Amino acids serve as building blocks, drugs and nutrients. The usual approach for analysis of amino acids include reversed-phase chromatography with an ion-pairing reagent, derivatization, and hydrophilic interaction chromatography (HILIC). Three amino acids (phenylalanine, tyrosine, and DOPA) were retained and separated in underivatized form in HILIC cation-exchange mode of core-shell mixed-mode HPLC column. This method is fast and reliable and provides base-line separation for all amino acids in the application. Method can be adopted for analysis of other amino acids. Coresep S PURCHASE LEARN ABOUT Coresep S Conditions of Experiment Column: Coresep S Separation Modes: HILIC and cation-exchange Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A Mobile Phase: 85 % ACN with 15 mM AmAc pH 5 Detection: UV 270, ELSD Sample: 0.3 mg/ml Injection: 3 uL Flow rate: 0.5-2 ml/min Analytes Class of compounds: Amino acid, Catecholamine, Drug, Neurotransmitter, Supplement Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic Compounds: Phenylalanine, Tyrosine, DOPA
