DOTA

HPLC Analysis of Zwitterions DOTA and DO3A on Amaze SA Mixed-Mode Column
Conditions of Experiment
Column: Amaze SA
Separation Modes: reveresed-phase, cation-exchanage
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: 10% ACN with 100 mM sodium phosphate pH 3
Detection: 210 nm
Sample: 2 mg/ml
Injection: 1 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Adulterants, Amines, Amino acid, Drug, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: DOTA, DO3A, Sodium ion
HPLC Analysis of DOTA and DO3A on Amaze HD HPLC Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x100 mm, 5 um, 100A
Mobile Phase: ACN with AmAc buffer pH 4
Detection: ELSD, 45°C
Sample: 0.5-0.8 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Adulterants, Amines, Amino acid, Drug, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Sodium ion, DOTA, DO3A
HPLC Analysis of DO3A and DOTA on Amaze TH HPLC Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x100 mm, 5 um, 100A
Mobile Phase: ACN with AmAc buffer pH 4
Detection: ELSD, 45°C
Sample: 0.5-0.8 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Adulterants, Amines, Amino acid, Drug, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Sodium ion, DOTA, DO3A