HPLC Analysis of Insecticide Methiocarb on Coresep 100 Mixed-Mode Column
Application description

Methiocarb is carbamate pesticide used as a bird repellent, insecticide, and acaricide. This and other carbamates are widely used in agriculture. Methiocarb is hydrophobic and neutral in nature. It was analyzed on Coresep 100 in reversed-phase mode. The compound is well retained with perfect peak shape. This robust method can be used for analysis of various pesticides. The method is fully compatible with mass spectrometry.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min with 3 min hold
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Class of compounds: Herbicide, Pesticide, Pyridine
Nature of compounds: Basic, Hydrophobic
Compounds: Dursban