Application description
This HPLC method is tailored for the analysis of the Trijardy diabetes drug combination, comprising Empagliflozin, Metformin, and Linagliptin, using an Amaze SC Mixed-Mode Column. The separation is conducted in both reversed-phase and cation-exchange modes, allowing for comprehensive characterization of the analytes.
Empagliflozin is a sodium-glucose cotransporter-2 (SGLT2) inhibitor used in the management of type 2 diabetes mellitus. It is a hydrophilic compound with moderate water solubility and contains a glycoside moiety, contributing to its polar nature.
Metformin is a biguanide antidiabetic medication widely prescribed to control blood sugar levels in individuals with type 2 diabetes. It is highly water-soluble and possesses basic properties due to the presence of amino groups. Metformin exhibits a moderate degree of polarity and interacts readily with acidic and basic functional groups.
Linagliptin is a dipeptidyl peptidase-4 (DPP-4) inhibitor used in the treatment of type 2 diabetes. It is sparingly soluble in water and exhibits moderate lipophilicity. Linagliptin contains a pyrimidine ring structure with multiple functional groups, contributing to its polar nature.
The Amaze SC Mixed-Mode Column is chosen for its dual functionality in reversed-phase and cation-exchange modes. In reversed-phase mode, the column facilitates separation based on differences in hydrophobicity, while in cation-exchange mode, it selectively retains and separates positively charged species, such as Metformin, based on ionic interactions.
By employing this HPLC method with the Amaze SC Mixed-Mode Column, precise quantification and characterization of the Trijardy diabetes drug combination can be achieved, providing valuable insights into their pharmaceutical formulations and therapeutic efficacy in managing diabetes.
Conditions of Experiment
| Column: |
Amaze SC |
| Separation Modes: |
reversed-phase, cation-exchange |
| Column Dimenstions: |
3x100 mm, 3 um, 100 A |
| Mobile Phase: |
ACN/water/ammonium phosphate |
| Detection: |
235 nm |
| Sample: |
0.2-0.2 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
0.6 ml/min |
Analytes
| Class of compounds: |
Drug |
| Nature of compounds: |
Basic, Hydrophilic, Hydrophobic, Neutral, Polar |
| Compounds: |
Empagliflozin, Metformin, Linagliptin |
