HPLC Separation of Four Catecholamines on Amaze SC Mixed-Mode Column with LC/MS Compatible Conditions
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase cation-exchange
Column Dimenstions: 3.0x100 mm, 5 μm, 100A
Mobile Phase: 40% ACN with 40 mM AmFm pH 3
Detection: UV 250 nm
Sample: 0.3 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Class of compounds: Aromatic base, Catecholamine, Drug, Neurotransmitter, Stimulant
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Epinephrine, Norepinephrine, Dopamine, Ephedrine
HPLC Separation of Hydrophobic, Hydrophilic, Basic and Acidic Compounds on Amaze TR Mixed-Mode Column
Conditions of Experiment
Column: Amaze TR
Separation Modes: RP, cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: 30% ACN with 30 mM AmAc pH 5
Detection: UV 275 nm
Sample: 0.2-0.3 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic base, Aromatic compound, Catecholamine, Drug, Neurotransmitter, Organic acid, Organic compound, Preservative, Stimulant
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: Norphenylephrine, Ephedrine, 3,4-Dihydroxybenzoic acid, Benzenesulfonic acid, Propylparaben, Propranolol