Application description
Escilatopram is an antidepressant medication mainly used to treat major depressive disorders. Escilatopram is a selective serotonin reuptake inhibitor. The compound is hydrophobic and basic in nature. HPLC method was developed for HPLC analysis of Escitalopram on Coresep 100 mixed-mode column. The method is robust with good peak shape for main compound. This method can be used for analysis of hydrophobic basic compounds in reversed-phase cation-exchange mode. Retention time is controlled by amount of ACN, buffer concentration and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of escitalopram in various samples. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Core-shell mixed-mode columns provide a great combination of unique selectivity, speed, and efficiency.
Conditions of Experiment
Column: |
Coresep 100 |
Separation Modes: |
reversed-phase and cation-exchange |
Column Dimenstions: |
3.0 x 100 mm |
Mobile Phase: |
65% ACN with 0.1% of H3PO4 |
Detection: |
UV 215 nm |
Sample: |
0.3 mg/ml |
Injection: |
1 uL |
Flow rate: |
0.6 mL/min |
Analytes
Class of compounds: |
Drug |
Nature of compounds: |
Basic, Hydrophobic |
Compounds: |
Escitalopram |