Ethylparaben

Ethylparaben C9H10O3

Select Applications HPLC Analysis of Eleven Compounds on Reversed-Phase Aromatic Phases. Relative Selectivity HPLC Analysis of Eleven Compounds on Amaze C18 SPF Column. Effect of Acetonitrile HPLC Analysis of Seven Aromatic Compounds on Reversed-Phase Aromatic Stationary Phases. Relative Selectivity HPLC Analysis of Seven Aromatic Compounds on Amaze C18 Reversed-Phase Column HPLC Analysis of Parabens on Heritage MA Mixed-Mode Column HPLC Analysis of Parabens on Amaze TR Mixed-Mode Column

HPLC Analysis of Eleven Compounds on Reversed-Phase Aromatic Phases. Relative Selectivity

Amaze C18 PURCHASE LEARN ABOUT Amaze C18

Conditions of Experiment

Column:	Amaze C18
Separation Modes:	reversed-phase
Column Dimenstions:	4.6x150 mm, 3 um, 100A
Mobile Phase:	ACN/Water/Ammonium phosphate/phosphoric acid
Detection:	215 nm
Sample:	0.2-0.4 mg/ml
Injection:	1 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Antibiotic, Aromatic base, Aromatic compound, Drug, Organic compound, Organic solvent, Preservative
Nature of compounds:	Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic
Compounds:	Ciprofloxacin, Pefloxacin, Propranolol, Diphenhydramine, Methylparaben, Dimethyl phthalate, Amitriptyline, Ethylparaben, Toluene, Sertraline, Propylparaben

HPLC Analysis of Eleven Compounds on Amaze C18 SPF Column. Effect of Acetonitrile

Amaze C18 PURCHASE LEARN ABOUT Amaze C18

Conditions of Experiment

Column:	Amaze C18
Separation Modes:	reversed-phase
Column Dimenstions:	4.6x150 mm, 3 um, 100A
Mobile Phase:	ACN/Water/Ammonium phosphate/phosphoric acid
Detection:	215 nm
Sample:	0.2-0.4 mg/ml
Injection:	1 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Antibiotic, Aromatic base, Aromatic compound, Drug, Organic compound, Organic solvent, Preservative
Nature of compounds:	Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic
Compounds:	Ciprofloxacin, Pefloxacin, Propranolol, Diphenhydramine, Methylparaben, Amitriptyline, Ethylparaben, Dimethyl phthalate, Sertraline, Propylparaben, Toluene

HPLC Analysis of Seven Aromatic Compounds on Reversed-Phase Aromatic Stationary Phases. Relative Selectivity

Amaze C18 PURCHASE LEARN ABOUT Amaze C18

Conditions of Experiment

Column:	Amaze C18
Separation Modes:	reversed-phase
Column Dimenstions:	4.6x150 mm, 3 um, 100A
Mobile Phase:	ACN/water/Ammonium phosphate
Detection:	220 nm
Sample:	0.3-0.5 mg/ml
Injection:	1 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Aldehyde, Aromatic acid, Aromatic compound, Organic acid, Organic compound, Preservative
Nature of compounds:	Acidic, Hydrophilic, Hydrophobic, Neutral
Compounds:	Benzyl alcohol, Phenol, Benzoic acid, Methylparaben, Benzonitrile, Ethylparaben, Propylparaben

HPLC Analysis of Seven Aromatic Compounds on Amaze C18 Reversed-Phase Column

Amaze C18 PURCHASE LEARN ABOUT Amaze C18

Conditions of Experiment

Column:	Amaze C18
Separation Modes:	reversed-phase
Column Dimenstions:	4.6x150 mm, 3 um, 100A
Mobile Phase:	ACN/water/Ammonium phosphate
Detection:	220 nm
Sample:	0.3-0.5 mg/ml
Injection:	1 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Aldehyde, Aromatic acid, Aromatic compound, Organic acid, Organic compound, Preservative
Nature of compounds:	Acidic, Hydrophilic, Hydrophobic, Neutral
Compounds:	Benzyl alcohol, Phenol, Benzoic acid, Methylparaben, Benzonitrile, Ethylparaben, Propylparaben

HPLC Analysis of Parabens on Heritage MA Mixed-Mode Column

Application description

Parabens are used as preservatives in food, beverages and drug formulations. Methyl, ethyl and propyl esters of 4-hydroxybenzoic acid are used in many formulations, mixtures and compositions as preservatives. Parabens are neutral in nature, and they are retained on Heritage HA mixed-mode column by pure reversed-phase mechanism. The method can be used in the analysis of parabens and other neutral or acidic preservatives by LC-UV and LC-MS. The retention time is controlled by the amount of organic component in the mobile phase. This method is robust and reproducible.

HPLC Analysis of Parabens on Heritage MA Mixed-Mode Column chromatogram

Heritage MA PURCHASE LEARN ABOUT Heritage MA

Conditions of Experiment

Column:	Heritage MA
Separation Modes:	reversed-phase
Column Dimenstions:	4.6x150 mm, 3 um, 100A
Mobile Phase:	60% ACN with 10 mM Am Ac ph 4
Detection:	UV 255 nm
Sample:	0.2 mg/ml
Injection:	3 ul
Flow rate:	1 ml/min

Analytes

Class of compounds:	Aromatic compound, Organic compound, Preservative
Nature of compounds:	Hydrophobic, Neutral
Compounds:	Methylparaben, Ethylparaben, Propylparaben

HPLC Analysis of Parabens on Amaze TR Mixed-Mode Column

Application description

Parabens are used as preservatives in food and drugs. Methyl, ethyl and propyl esters of 4-hydroxybenzoic acid are used in many formulations, mixtures and compositions. Parabens are neutral and hydrophobic in nature, and they are retained on a Heritage HA mixed-mode column by pure reversed-phase mechanism. The method can be used in the analysis of parabens and other neutral or acidic preservatives by LC-UV and LC-MS. The retention time is controlled by the amount of organic component of the mobile phase. This method is robust and reproducible.

HPLC Analysis of Parabens on Amaze TR Mixed-Mode Column chromatogram

Heritage MA PURCHASE LEARN ABOUT Heritage MA

Conditions of Experiment

Column:	Heritage MA
Separation Modes:	reversed-phase
Column Dimenstions:	4.6x150 mm, 3 um, 100A
Mobile Phase:	60% ACN with 10 mM Am Ac ph 4
Detection:	UV 255 nm
Sample:	0.2 mg/ml
Injection:	3 ul
Flow rate:	1 ml/min

Analytes

Class of compounds:	Aromatic compound, Organic compound, Preservative
Nature of compounds:	Hydrophobic, Neutral
Compounds:	Methylparaben, Ethylparaben, Propylparaben