Fosfomycin
The method involves the High-Performance Liquid Chromatography (HPLC) analysis of fosfomycin, TRIS (Tris(hydroxymethyl)aminomethane), and sodium ion using an Evaporative Light Scattering Detector (ELSD) on an Amaze TH Mixed-Mode Column. Fosfomycin is a broad-spectrum antibiotic with a phosphonic acid structure, used to treat various bacterial infections. It is water-soluble and stable under normal laboratory conditions. By employing this method, accurate quantification of Fosfomycin, TRIS, and sodium ion can be achieved, facilitating their analysis in pharmaceutical formulations, biological samples, and related research applications.
Column: | Amaze TH |
Separation Modes: | HILIC, cation-exchange, Anion-exchange |
Column Dimenstions: | 4.6x150 mm, 3 um, 100 A |
Mobile Phase: | 80% ACN with 20 mM ammonium formate pH 3 |
Detection: | ELSD, 45*C |
Sample: | various-0.5 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Amines, Antibiotic, Counterion, Drug, Inorganic cation |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Fosfomycin, TRIS, Sodium ion |
Column: | Amaze HA |
Separation Modes: | anion-exchange |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | 75% ACN with 15 mM AmFm pH 3.8 |
Detection: | Corona CAD |
Sample: | 0.1 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Antibiotic, Drug |
Nature of compounds: | Acidic, Hydrophilic |
Compounds: | Fosfomycin |
Sucrose, fosfomycin, sodium and TRIS are part of the fosfomycin formulation. Sucrose, fosfomycin, sodium and tris are very polar in nature. Fosfomycin is a broad-spectrum antibiotic which contains an epoxy fragment as well as phosphonic acid. This makes this drug very polar and acidic. Sodium ion and tromethamine are used as counter-ions for this polar drug molecule. All four compounds are different in nature - sucrose is polar and neutral, sodium is polar and basic, TRIS is polar and basic, and fosfomycin is polar and acidic. It is hard to retain and separate these compounds in RP chromatography. HILIC can retain these polar compounds and mixed-mode HILIC on the Amaze TH column allows to introduce cation- and anion-exchange mechanisms for better retention and better retention control. The presence of ionic interaction, in addition to HILIC, allows better control of elution and retention. The retention time can be controlled by three parameters - amount of acetonitrile, amount of buffer and the buffer pH. This method can be used for the analysis of drug formulation and level of fosfomycin in biofluids (serum, blood, urine, etc). The method is robust, reproducible and compatible with all major detection techniques.
Column: | Amaze TH |
Separation Modes: | HILIC, cation-exchange, anion-exchange |
Column Dimenstions: | 4.6x150 mm 5 um, 100A |
Mobile Phase: | ACN/water/Buffer |
Detection: | ELSD |
Sample: | 1 mg/ml |
Injection: | 10 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Amines, Antibiotic, Drug, Inorganic cation, Sugar |
Nature of compounds: | Acidic, Basic, Hydrophilic, Neutral, Polar |
Compounds: | Fosfomycin, Sucrose, Sodium ion, Tromethamine |
Column: | Amaze TH |
Separation Modes: | cation-exchange, anion-exchange |
Column Dimenstions: | 4.6x150 mm 5 um, 100A |
Mobile Phase: | 40% ACN with 20 mM AmAc pH 4 |
Detection: | ELSD |
Sample: | 1 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Amines, Antibiotic, Drug, Inorganic cation |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Fosfomycin, Sodium ion, Tromethamine |