Gluconic acid
Column: | Amaze TH |
Separation Modes: | HILIC, anion-exchange, cations-exchange |
Column Dimenstions: | 4.6x150 mm, 5 um, 100A |
Mobile Phase: | 80% ACN with 20 mM AmFm pH 3 |
Detection: | ELSD |
Sample: | 0.3-0.5 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Inorganic cation, Organic acid, Sugar |
Nature of compounds: | Acidic, Basic, Hydrophilic, Neutral, Polar |
Compounds: | Glucose, Gluconic acid, Glucuronic acid, Sodium ion |
Column: | Amaze HD |
Separation Modes: | HILIC, anion-exclusion |
Column Dimenstions: | 4.6x150 mm, 3 um , 100A |
Mobile Phase: | 90% ACN witj 0.05% phosphoric acid |
Detection: | 205 nm |
Sample: | 0.3-6 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Organic acid, Sugar |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Gluconic acid, Saccharic acid |
Column: | Heritage MA |
Separation Modes: | reversed-phase, anion-exchange |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | 2% ACN with 0.05% phosphoric acid |
Detection: | 205 nm |
Sample: | 0.3-6 mg/ml |
Injection: | 2 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Organic acid, Sugar |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Gluconic acid, Saccharic acid |
Several techniques are used for the retention and separation of polar and polar ionizable compounds. The HILIC mixed-mode proves to be a powerful approach to independently control elution of ionizable compounds by changing buffer concentration and buffer pH. Glucose is a six member monosaccharide which is polar and neutral in nature. Gluconic acid is a polar and acidic sugar-based compound which is a product of glucose oxidation in nature. Gluconic acid naturally occurs in fruits, honey and wine. It is also used as a food additive to regulate acidity. Gluconic acid mostly exists in a linear form. Glucuronic acid is a cyclic sugar-based acid which is polar and acidic in nature. Glucuronic acid is used as a building block for proteoglycans and glycoglycerolipids. Glucuronic and gluconic acids are fermentation products in Kombucha tea. We have developed a HILIC/anion-exchange mixed-mode approach for the separation of neutral and acidic sugars.Sugars are retained by an HILIC mechanism and sugar-based acids are retained by HILIC and anion-exchange mechanisms. The retention time is controlled by the amount of ACN, buffer pH and buffer concentration. This method is robust and reproducible. It can be used for analysis of neutral acidic and basic sugar-like molecules in one run. This method is compatible with major detection techniques ( (ELSD, CAD, RI, UV and mass spec)
Column: | Amaze TH |
Separation Modes: | HILIC, anion-exchnage |
Column Dimenstions: | 4.6x150 mm, 5 um, 100A |
Mobile Phase: | ACN/water/AmAc Buffer |
Detection: | ELSD, 50°C |
Sample: | 1 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Organic acid, Sugar |
Nature of compounds: | Acidic, Hydrophilic, Neutral, Polar |
Compounds: | Glucose, Gluconic acid, Glucuronic acid |
Several techniques are used for the retention and separation of polar and polar ionizable compounds. HILIC mixed-mode proves to be a powerful approach to independently control the elution of ionizable compounds by changing buffer concentration and buffer pH. Glucose is a six member monosaccharide which is polar and neutral in nature. Gluconic acid is polar and an acidic sugar-based compound which is a product of glucose oxidation in nature. Gluconic acid naturally occurs in fruits, honey and wine. It is also used as a food additive to regulate acidity. Gluconic acid mostly exists in a linear form. Gluconic acid is one of the fermentation products in Kombucha tea. We have developed a HILIC/anion-exchange mixed-mode approach for the separation of glucose and gluconic acid on the Amaze TH mixed-mode HPLC column. Glucose is retained by the HILIC mechanism and gluconic acid is retained by HILIC and anion-exchange mechanisms. The retention time is controlled by the amount of ACN, buffer pH and buffer concentration. The change in pH will affect ionization and the polarity of the stationary phase and ionizable analytes. This method is robust and reproducible. It can be used for the analysis of neutral acidic and basic sugar-like molecules in one run. This method is compatible with major detection techniques ( (ELSD, CAD, RI, UV and mass spec)
Column: | Amaze TH |
Separation Modes: | HILIC, anion-exchnage |
Column Dimenstions: | 4.6x150 mm, 5 um, 100A |
Mobile Phase: | ACN/water/AmAc Buffer |
Detection: | ELSD, 50°C |
Sample: | 1 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Organic acid, Sugar |
Nature of compounds: | Acidic, Hydrophilic, Neutral, Polar |
Compounds: | Glucose, Gluconic acid |