HPLC Analysis of Drug Guaifenesin on Heritage C18 Column According to US Pharmacopeia
Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: MeOH/H2O/AcOH 40/60/1
Detection: UV 254 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Class of compounds: Aromatic acid, Aromatic compound, Drug, Organic acid
Nature of compounds: Acidic, Hydrophilic, Neutral, Polar
Compounds: Guaifenesin, Benzoic acid
Fast HPLC Analysis of Drug Composition on Coresep 100 Mixed-Mode Core-Shell Column
Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: MeCN 15% with 0.1% TFA
Detection: UV 275 nm
Sample: 0.1-0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Amino acid, Aromatic compound, Drug, Supplement
Nature of compounds: Hydrophilic, Neutral, Polar, Zwitterionic
Compounds: Phenylalanine, Acetaminophen, Guaifenesin, Guaiacol