Guaifenesin

Cheratussin AC is a combination medicine used to treat cough and chest congestion caused by allergies, the common cold, or the flu. Cheratussin AC will not treat a cough that is caused by smoking, asthma, or emphysema. The active ingredients are guaifenesin and codeine. Codeine is a narcotic cough suppressant. It affects the signals in the brain that trigger cough reflex. Guaifenesin is an expectorant. It helps loosen congestion in your chest and throat, making it easier to cough out through your mouth. Cheratussin AC is a combination medicine used to treat cough and chest congestion caused by allergies, the common cold, or the flu. Cheratussin AC will not treat a cough that is caused by smoking, asthma, or emphysema. Codeine is a highly hydrophobic and basic compound and guaifenesin is a neutral hydrophilic compound. The highly effective separation was achieved on a Coresep 100 mixed-mode HPLC column. The column is compatible with 100% ACN and 100% water due to the presence of hydrophilic ionizable groups on the surface of core-shell particles. Coresep 100 resist phase collapse which is typical for pure reversed-phase columns. Method and Coresep 100 column can be used for the analysis of various drugs and drug composition where alternative selectivity is required to achieve baseline separation. Method and column can be easily validated. Results are reproducible and robust

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Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase, cation-exchange
Column Dimenstions:	4.6x150 mm, 2.7 um, 90A
Mobile Phase:	A: 15% ACN with 0.02% H2SO4 B: 50% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold
Detection:	UV 275
Sample:	various
Injection:	3 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Aromatic base, Aromatic compound, Controlled substance, Drug
Nature of compounds:	Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds:	Guaifenesin, Codeine

Mucinex Fast-Max Severe Congestion & Cough is a combination medicine used to treat cough, stuffy nose, and chest congestion caused by allergies, the common cold, or the flu. It is a complex mixture of several active and inactive ingredients. Active ingredients of this OTC drug composition are phenylephrine, guaifenesin, and dextromethorphan. Phenylephrine is a hydrophilic basic drug, which is primarily used as a decongestant, to dilate the pupil, to increase blood pressure, and to relieve hemorrhoids. Guaifenesin is a hydrophilic neutral compound which is added to cough and cold formulations to help with coughing thick mucus it is used in combination with dextromethorphan to alleviate symptoms related to cold, flu and congestion. Dextromethorphan is a hydrophobic and basic compound, which serves as a cough suppressant in over-the-counter (OTC) medications. Guaifenesin is retained by a pure reversed-phase mechanism, and dextromethorphan is retained by a combination of reversed-phase and cations-exchange mechanisms. Inactive ingredients consist of croscarmellose sodium, FD&C blue no. 2 aluminum lake, FD&C red no. 40 aluminum lake, methacrylic acid ethyl acrylate copolymer, mica, microcrystalline cellulose, polyethylene glycol 3350, polysorbate 80, polyvinyl alcohol, povidone K29/32, sodium bicarbonate, talc, titanium dioxide. Baseline separation of active and inactive ingredients was achieved on Coresep 100 mixed-mode column. Coresep 100 column provides good retention of basic compounds without the use of ion-pairing reagents and high pH. It retains compounds of different natures by a combination of reversed-phase, cation-exchange, and anion-exclusion mechanisms. The retention time of the robust separation is controlled by the amount of ACN, buffer concentration, and buffer. Various buffers and additives to the mobile phase can be used to control retention and be suitable with a chosen detection technique. This method and HPLC column can be used to achieve highly efficient, robust baseline separation of complex mixtures.

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Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase, cation-exchange
Column Dimenstions:	4.6x150 mm, 2.7 um, 90A
Mobile Phase:	A: 15% ACN with 0.02% H2SO4 B: 50% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold
Detection:	UV 275
Sample:	various
Injection:	3 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Amines, Aromatic base, Aromatic compound, Catecholamine, Drug, Neurotransmitter
Nature of compounds:	Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds:	Phenylephrine, Guaifenesin, Dextromethorphan

Guaifenesin is medication which is used to help with clearing airways from phlegm and mucus. It is a relatively hydrophobic and neutral compound. We have developed a method for analysis of guaifenesin and related impurities in various drug formulations. The compound is retained on a Coresep 100 column by a pure reversed-phase mechanism. Retention time is controlled by the amount of acetonitrile in the mobile phase. Buffer selection depends on the detection technique used to monitor guaifenesin and related impurities. Coresep 100 HPLC column is a reversed-phase cation-exchange column, based on core-shell particles. It demonstrates very high efficiency and robustness as well as a unique alternative to RP and HILIC selectivity.

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Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase, cation-exchange
Column Dimenstions:	4.6x150 mm, 2.7 um, 90A
Mobile Phase:	A: 15% ACN with 0.02% H2SO4 B: 50% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold
Detection:	UV 255
Sample:	2 mg/ml
Injection:	3 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Aromatic acid, Aromatic base, Aromatic compound, Drug
Nature of compounds:	Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds:	Guaifenesin, Related Impurities

Robitussin Severe Cough, Cold & Flu Formulation is a complex mixture of organic and inorganic compounds. The main active drug ingredients are acetaminophen, phenylephrine, guaifenesin, and dextromethorphan. Inactive ingredients include anhydrous citric acid, edetate disodium, FD&C red no. 40, glycerin, menthol, natural and artificial flavors, polyethylene glycol, propyl gallate, propylene glycol, purified water, sodium benzoate, sodium citrate, sorbitol solution, sucralose, triacetin, xanthan gum. Acetaminophen or paracetamol is a relatively hydrophilic drug, which is used to treat pain and fever. Phenylephrine is a hydrophilic basic drug, which is primarily used as a decongestant, to dilate the pupil, to increase blood pressure, and to relieve hemorrhoids. Guaifenesin is a hydrophilic organic compound which is used as a drug that is used to help to remove mucus from the mouth due to the common cold and flu. Dextromethorphan is a hydrophobic basic compound, which is used as an over-the-counter cough suppressant. Excipients are hydrophilic and hydrophobic in nature and are added to improve taste and increase stability of the formulation. Several complex methods are required to fully analyze this drug composition. We have developed a simple UV method using Coresep 100 mixed-mode column. Coresep 100 column is a reversed-phase cation-exchange column, based on 2.7 um core-shell articles. It is designed to retain various analytes without the use of ion-pairing reagents. This approach makes these columns fully compatible with mass spec detection. The method for analysis of this drug formulation is reproducible and robust and provides baseline separation of hydrophobic basic, hydrophilic basic, neutral, hydrophobic, and hydrophobic acidic compounds. Hydrophilic acidic compounds might not be retained. The column is compatible with 100% water and 100% ACN and totally resistant to phase collapse, due to polar-embedded ionizable groups on the surface of core-shell particles. Mixed-mode HPLC columns provide 2-D selectivity for the retention of hydrophobic and hydrophilic ionizable compounds.

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Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase, cation-exchange
Column Dimenstions:	4.6x150 mm, 2.7 um, 90A
Mobile Phase:	"A: 10% ACN with 0.03% H2SO4 B: 55% ACN with 0.25% H2SO4 from 100% A to 100% B in 8 min, 4 min hold "
Detection:	UV 275
Sample:	various
Injection:	3 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Amines, Aromatic base, Aromatic compound, Catecholamine, Drug, Neurotransmitter
Nature of compounds:	Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds:	Acetaminophen, Phenylephrine, Guaifenesin, Dextromethorphan

Tylenol Severe Cold and Flu formulation includes active ingredients as well as excipients. Active components include acetaminophen, phenylephrine, guaifenesin, and dextromethorphan. Inactive ingredients include carnauba wax, croscarmellose sodium, D&C yellow no. 10 aluminum lake, flavor, hydroxypropyl cellulose, hypromellose, magnesium stearate, microcrystalline cellulose, polyethylene glycol, pregelatinized starch, sucralose, titanium dioxide. The complex mixture of hydrophobic, hydrophilic, basic, acidic, and neutral components of Tylenol medication was fully separated on Coresep 100 mixed-mode core-shell reversed-phase cation-exchange column. The method is robust and reproducible and can be used for the analysis of drugs and drug compositions in various sample matrices. Various buffers and additives to the mobile phase can be used depending on the detection technique. The retention time of active ingredients, impurities, and excipients is controlled by the amount of acetonitrile (ACN), buffer pH, and buffer concentration. Core-shell columns provide very high efficiency of separation and mixed-mode provides unique selectivity and ability to retain hydrophilic compounds without the use of ion-pairing reagents. Mixed-mode columns are compatible with 100% ACN and 100% water due to the presence of polar and ionizable groups on the surface of silica gel. The column can be effectively used in isocratic and gradient runs to achieve baseline resolution between various active and inactive ingredients.

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Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase, cation-exchange
Column Dimenstions:	4.6x150 mm, 2.7 um, 90A
Mobile Phase:	"A: 10% ACN with 0.03% H2SO4 B: 70% ACN with 0.25% H2SO4 from 100% A to 100% B in 8 min, 4 min hold "
Detection:	UV 275
Sample:	various
Injection:	3 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Amines, Aromatic base, Aromatic compound, Catecholamine, Drug, Neurotransmitter
Nature of compounds:	Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds:	Acetaminophen, Phenylephrine, Guaifenesin, Dextromethorphan

Tussin Cough and Cold formulation consists of medication to relieve cough caused by the common cold, bronchitis, or other respiratory issues. The active ingredients include guaifenesin and dextromethorphan. Guaifenesin belongs to a class of drugs known as expectorants. It helps to deal with mucus, clearing congestion and making breathing easier. Dextromethorphan belongs to a class of drugs known as a cough suppressant, it works on the part of the brain to reduce the urge to cough. Inactive ingredients include anhydrous citric acid, FD&C red 40, flavor, glucose, glycerin, high fructose corn syrup, menthol, purified water, saccharin sodium, sodium benzoate. Active ingredients and excipients were separated on Coresep 100 mixed-mode reversed-phase cation-exchange column with a simple gradient. Baseline separation was achieved under 10 minutes and showed perfect peak shape and robustness. The method uses ACN/water/sulfuric acid to elute all compounds. Other buffers and additives can be used to retain and separate hydrophobic, hydrophilic, neutral, and basic compounds. Other buffers and additives can be used to replace sulfuric acid and accommodate detection techniques where the volatile mobile phase is required (ELSD, CAD, mass-spectrometry). The retention time of active ingredients, impurities, and excipients is controlled by the amount of acetonitrile (ACN), buffer pH, and buffer concentration. Core-shell columns provide very high efficiency of separation and mixed-mode provides unique selectivity and ability to retain hydrophilic compounds without the use of ion-pairing reagents.

Coresep 100 PURCHASE LEARN ABOUT Coresep 100

Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase, cation-exchange
Column Dimenstions:	4.6x150 mm, 2.7 um, 90A
Mobile Phase:	"A: 10% ACN with 0.02% H2SO4 B: 70% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold "
Detection:	UV 275
Sample:	various
Injection:	3 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Amines, Aromatic base, Aromatic compound, Drug
Nature of compounds:	Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds:	Guaifenesin, Dextromethorphan

Cough, cold and flu medications are a complex mixture of active ingredients and excipients. It usually contains hydrophilic and hydrophobic drugs, counterions, other organic and inorganic additives. Analysis of such complex mixtures are a challenging task. Analysis is usually done with several methods, including reversed-phase and ion-exchange chromatography. Active ingredients of Wal-Tussin DM MAX (guaifenesin and dextromethorphan and excipients were separated on Coresep 100 mixed-mode reversed-phase and cation-exchange HPLC column. Guaifenesin is a hydrophilic neutral compound which is added to cough and cold formulations to help with coughing thick mucus it is used in combination with dextromethorphan to alleviate symptoms related to cold, flu and congestion. Dextromethorphan is a hydrophobic and basic compound, which serves as a cough suppressant in over-the-counter (OTC) medications. Guaifenesin is retained by a pure reversed-phase mechanism, and dextromethorphan is retained by a combination of reversed-phase and cations-exchange mechanisms. This HPLC method demonstrates baseline separation and robustness in analysis of cough and cold formulations. Coresep 100 columns can be successfully used for analysis of various compounds, drug compositions and formulations. The column is compatible with 100% water and 100% ACN and can be used in isocratic or various gradient modes. The retention of compounds is adjusted by changing the amount of ACN, buffer pH, and buffer concentration. The method can be applied to the analysis of drugs in biofluids. Most common detection techniques can be used (UV, ELSD, mass-spec, etc.). Core-shell columns provide very high efficiency of separation and mixed-mode provides unique selectivity and ability to retain hydrophilic compounds without the use of ion-pairing reagents.

Coresep 100 PURCHASE LEARN ABOUT Coresep 100

Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase, cation-exchange
Column Dimenstions:	4.6x150 mm, 2.7 um, 90A
Mobile Phase:	A: 10% ACN with 0.02% H2SO4 B: 70% ACN with 0.2% H2SO4 from 100% A to 100% B in 8 min, 4 min hold
Detection:	UV 275
Sample:	various
Injection:	3 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Amines, Aromatic base, Aromatic compound, Drug
Nature of compounds:	Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds:	Guaifenesin, Dextromethorphan