HEPES
HPLC Separation of CAPS and HEPES Biological Buffers on Amaze TH Mixed-Mode Column
Conditions of Experiment
| Column: | Amaze TH |
| Separation Modes: | HILIC, cation-exchange |
| Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
| Mobile Phase: | ACN/water/ammonium formate |
| Detection: | ELSD, 45*C |
| Sample: | 1 mg/ml |
| Injection: | 3 ul |
| Flow rate: | 0.6 ml/min |
Analytes
| Class of compounds: | Amines, Amino acid, Biological buffer, Inorganic cation |
| Nature of compounds: | Basic, Hydrophilic, Polar, Zwitterionic |
| Compounds: | CAPS, Sodium ion, HEPES |
