Heroin is an opioid recreational illegal drug. It is Schedule I controlled substance. Heroin is hydrophobic and basic in nature. It is retained on mixed-mode Coresep 100 HPLC column by combination of strong hydrophobic and strong cation-exchange mechanisms. This method is robust, fast and reliable with good retention and peak shape for highly hydrophobic and basic molecule. Retention time is controlled by the amount of acetonitrile, buffer concentration, and buffer pH. The method can be applied to the analysis of other illegal drugs in biofluids and formulations. The method is isocratic and fully compatible with LC/MS.
|Separation Modes:||reversed-phase and cation-exchange|
|Column Dimenstions:||3.0 x 100 mm|
|Mobile Phase:||60% CAN with 30 mM AmFm pH 3|
|Detection:||UV 275 nm|
|Flow rate:||0.8 mL/min|
|Class of compounds:||Controlled substance, Illegal drug|
|Nature of compounds:||Basic, Hydrophobic|