Histidine
Histidine
Histidine
Select Applications
HPLC Analysis of Sucrose, Histidine and Arginine on Amaze HD Mixed-Mode Column
HPLC Analysis of 20 Amino Acids on Amaze SC Mixed-Mode Column in RP and Cation-exchange Modes
HPLC Analysis of Sucrose, Histidine and Arginine on Amaze HD Mixed-Mode Column
HPLC Analysis of Histidine and Related Impurities
HPLC Analysis of Sucrose, Histidine and Arginine on Amaze HD Mixed-Mode Column
Conditions of Experiment
Column: | Amaze HD |
Separation Modes: | HILIC, cation-exchange, anion-exclusion |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | 75% ACN with 50 mM AmAc pH 5 |
Detection: | ELSD |
Sample: | 0.2-0.5 mg/ml |
Injection: | 2 uL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Amino acid, Inorganic anion, Sugar, Supplement |
Nature of compounds: | Acidic, Hydrophilic, Neutral, Polar, Zwitterionic |
Compounds: | Chloride Ion, Sucrose, Histidine, Arginine |
HPLC Analysis of 20 Amino Acids on Amaze SC Mixed-Mode Column in RP and Cation-exchange Modes
Conditions of Experiment
Column: | Amaze SC |
Separation Modes: | reversed-phase cation-exchange |
Column Dimenstions: | 4.6x250 mm, 5 μm, 100A |
Mobile Phase: | A: 2% ACN with 0.1% TFA B: 25% ACN with 0.3% TFA from 100% A to 100% B in 20 min, 8 min hold |
Detection: | Corona CAD |
Sample: | 0.2 mg/ml |
Injection: | 5 μL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Amino acid, Neurotransmitter, Supplement |
Nature of compounds: | Acidic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Aspartic acid, Serine, Asparagine, Cysteine, Glutamic acid, Threonine, Glutamine, Glycine, Alanine, Proline, Valine, Methionine, Tyrosine, Isoleucine, Leucine, Phenylalanine, Lysine, Histidine, Tryptophan, Arginine |
HPLC Analysis of Sucrose, Histidine and Arginine on Amaze HD Mixed-Mode Column
Conditions of Experiment
Column: | Amaze HD |
Separation Modes: | HILIC, cation-exchange, anion-exclusion |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | 75% ACN with 50 mM AmAc pH 5 |
Detection: | ELSD, 40°C |
Sample: | 2 mg/ml |
Injection: | 3 uL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Amino acid, Inorganic anion, Sugar, Supplement |
Nature of compounds: | Acidic, Hydrophilic, Neutral, Polar, Zwitterionic |
Compounds: | Chloride Ion, Sucrose, Histidine, Arginine |