Histidine

Histidine C6H9N3O2

Histidine

Applications

HPLC Analysis of Histidine and Related Impurities

HPLC Analysis of Histidine and Related Impurities chromatogram
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
Column Dimenstions: 4.6 x 150 mm, 2.7 um, 90A
Mobile Phase: ACN from 2% to 20%, Na2HPO4 pH 6 from 10 mM to 50 mM in 12 min
Detection: UV 210 nm
Sample:
Injection:
Flow rate: 1 mL/min
Analytes
1. Alanine
2. Histidine
3. Lysine
4. Arginine

Class of compounds: Amino acid, Supplement
Nature of compounds: Hydrophilic, Polar, Zwitterionic

Application description

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