Homovanillic acid

Ultra-Fast HPLC Analysis of Aromatic Hydrophilic Acids on Coresep SB Column in Reversed-Phase and Anion-Exchange Modes
Application description

Homovanillic and vanilmandelic acid are major catecholamine pathway metabolites. VMA and HVA can be found in urine along with other metabolites. Homovanillic and vanilmandelic acids were separated by combination of weak reversed-phase and anion-exchange mechanisms on the core-shell column. This method can be used for analysis of hydrophilic acidic compounds with LC. MS compatible conditions. Both compounds elute with a good peak shape. Retention time is controlled by amount of acetonitrile, buffer concentration, and buffer pH. Stationary phase does not change its ionization state with the change of buffer pH, but ionization state of acidic compounds is greatly affected by buffer pH.

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: 50% ACN with 50 mM sodium phosphate (monobasic) pH 3
Detection: UV 230 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.8 ml/mim
Class of compounds: Aromatic acid, Catecholamine, Neurotransmitter, Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Homovanillic acid, Vanilmandelic acid