Application description
Hydrazine is a highly polar and toxic compound which is used as a precursor to pesticides and pharmaceuticals. It is used in coupling reactions as well as hetero atom-containing rings such as pyrazoles and pyridazines. It is also used as a part of agricultural formulations. Due to its toxicity, a rapid and accurate determination of hydrazine in various products and matrices is required. Hydrazine, due to its polar nature, has no retention on reversed-phase columns. We have developed a fast and robust HPLC-ELSD method for the determination of hydrazine. It is retained on the Coresep 100 mixed-mode cation-exchange column by cation-exchange mechanism. Various mobile phases can be used to facilitate the retention of hydrazine. The replacement of trifluoroacetic acid with formic acid or ammonium formate makes this method MS-compatible.
Conditions of Experiment
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase, cation-exchange |
| Column Dimenstions: |
4.6x150 mm, 2.7 um, 90A |
| Mobile Phase: |
aqeous TFA |
| Detection: |
ELSD, 40°C |
| Sample: |
0.5 mg/ml |
| Injection: |
5 uL |
| Flow rate: |
1 ml/min |
Analytes
| Class of compounds: |
Amines, Organic compound |
| Nature of compounds: |
Basic, Hydrophilic, Polar |
| Compounds: |
Hydrazine |
