Hydrazine is a highly polar and toxic compound which is used as a precursor to pesticides and pharmaceuticals. It is used in coupling reactions as well as hetero atom-containing rings such as pyrazoles and pyridazines. It is also used as a part of agricultural formulations. Due to its toxicity, a rapid and accurate determination of hydrazine in various products and matrices is required. Hydrazine, due to its polar nature, has no retention on reversed-phase columns. We have developed a fast and robust HPLC-ELSD method for the determination of hydrazine. It is retained on the Coresep 100 mixed-mode cation-exchange column by cation-exchange mechanism. Various mobile phases can be used to facilitate the retention of hydrazine. The replacement of trifluoroacetic acid with formic acid or ammonium formate makes this method MS-compatible.
|Separation Modes:||reversed-phase, cation-exchange|
|Column Dimenstions:||4.6x150 mm, 2.7 um, 90A|
|Mobile Phase:||aqeous TFA|
|Flow rate:||1 ml/min|
|Class of compounds:||Amines, Organic compound|
|Nature of compounds:||Basic, Hydrophilic, Polar|