Application description
This HPLC method details the separation of Ibandronic acid, Etidronic acid, and Zoledronic acid using an Amaze HD Column, employing both Hydrophilic Interaction Liquid Chromatography (HILIC) and anion-exclusion modes for optimal separation.
Ibandronic Acid: Ibandronic acid belongs to the bisphosphonate class of drugs used in the treatment of osteoporosis and bone metastasis. It is highly water-soluble and contains two phosphonic acid groups, which impart strong acidic properties.
Etidronic Acid: Etidronic acid is another bisphosphonate compound utilized in the treatment of various bone disorders. It is water-soluble and possesses phosphonic acid functional groups, contributing to its acidic nature and interaction with chromatographic stationary phases.
Zoledronic Acid: Zoledronic acid is a potent bisphosphonate drug used for the treatment of osteoporosis and bone-related conditions. It exhibits high water solubility and contains phosphonic acid groups, rendering it acidic and suitable for chromatographic separation.
The Amaze HD Column is chosen for its ability to handle polar and charged compounds effectively. In HILIC mode, the column facilitates retention and separation based on the hydrophilicity of the analytes, while in anion-exclusion mode, it selectively excludes negatively charged species, allowing for separation based on differences in charge and size.
By utilizing this HPLC method, precise separation and quantification of Ibandronic, Etidronic, and Zoledronic acids can be achieved, facilitating their analysis in pharmaceutical formulations and biomedical research.
Conditions of Experiment
| Column: |
Amaze HD |
| Separation Modes: |
HILIC, anion-exclusion |
| Column Dimenstions: |
3x100 mm, 3 um, 100 A |
| Mobile Phase: |
ACN/water/ammonium formate pH 3 |
| Detection: |
ELSD, 45*C |
| Sample: |
various-0.5 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
0.6 ml/min |
Analytes
| Class of compounds: |
Drug, Organic acid |
| Nature of compounds: |
Acidic, Hydrophilic, Polar |
| Compounds: |
Ibandronic acid, Etidronic acid, Zoledronic acid |
