HPLC Separation of Nucleobases and Nucleosides on Amaze TCH HILIC Mixed-Mode Column
Conditions of Experiment
Column: Amaze TCH
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: ACN from 92% to 70% in 15 min, 10 mM AmAc pH 5
Detection: UV 255 nm
Sample: 0.2-0.5 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic base, Nucleobase, Nucleoside
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Thymine, Uracil, Thymidine, Uridine, Adenine, Adenosine, Cytosine, Inosine, Guanine, Cytidine, Guanosine
HPLC Method for Analysis of Inosine and Deoxyinosine on Amaze HD Column
Application description

Inosine is a nucleoside containing hypoxanthine and ribose molecules. Deoxyinosine is a nucleoside containing hypoxanthine and deoxyribose molecules. Both molecules are polar and slightly basic. Mobile phase allows to use mass spectrometry detection technique as well as UV, ELSD, and CAD.

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 3.2 x 50 mm
Mobile Phase: MeCN/MeOH (97/3) with 0.1% HCOOH and 0.01% AmFm
Detection: 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1.0 mL/min
Class of compounds: Nucleoside
Nature of compounds: Hydrophilic, Neutral, Polar
Compounds: 2′-Deoxyadenosine, Inosine