Inosine Inosine Inosine Select Applications HPLC Separation of Nucleobases and Nucleosides on Amaze TCH HILIC Mixed-Mode Column HPLC Method for Analysis of Inosine and Deoxyinosine on Amaze HD Column HPLC Separation of Nucleobases and Nucleosides on Amaze TCH HILIC Mixed-Mode Column Amaze TCH PURCHASE LEARN ABOUT Amaze TCH Conditions of Experiment Column: Amaze TCH Separation Modes: HILIC, cation-exchange Column Dimenstions: 4.6x150 mm, 3 um, 100A Mobile Phase: ACN from 92% to 70% in 15 min, 10 mM AmAc pH 5 Detection: UV 255 nm Sample: 0.2-0.5 mg/ml Injection: 2 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic base, Nucleobase, Nucleoside Nature of compounds: Basic, Hydrophilic, Neutral, Polar Compounds: Thymine, Uracil, Thymidine, Uridine, Adenine, Adenosine, Cytosine, Inosine, Guanine, Cytidine, Guanosine HPLC Method for Analysis of Inosine and Deoxyinosine on Amaze HD Column Application description Inosine is a nucleoside containing hypoxanthine and ribose molecules. Deoxyinosine is a nucleoside containing hypoxanthine and deoxyribose molecules. Both molecules are polar and slightly basic. Mobile phase allows to use mass spectrometry detection technique as well as UV, ELSD, and CAD. Amaze HD PURCHASE LEARN ABOUT Amaze HD Conditions of Experiment Column: Amaze HD Separation Modes: HILIC Column Dimenstions: 3.2 x 50 mm Mobile Phase: MeCN/MeOH (97/3) with 0.1% HCOOH and 0.01% AmFm Detection: 275 nm Sample: 0.3 mg/ml Injection: 1 uL Flow rate: 1.0 mL/min Analytes Class of compounds: Nucleoside Nature of compounds: Hydrophilic, Neutral, Polar Compounds: 2′-Deoxyadenosine, Inosine
