Iodide ion

HPLC Analysis of Divalent Cations and Corresponding Counterions on Amaze TCH Mixed-Mode Column
Conditions of Experiment
Column: Amaze TCH
Separation Modes: HILIC, anion-exchange, cations-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: ACN
from 75% to 30%, AmFm from 40 mM pH 4.0 to 40 mM pH 2.5
Detection: ELSD
Sample: 0.3-0.5 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Contaminant, Inorganic acid, Inorganic anion, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Iodide ion, Nitrate ion, Bromide ion, Bromate Ion, Calcium ion, Persulfate ion, Magnesium ion, Phosphate Ion, Nickel ion, Copper ion, Zinc ion, Barium ion
HPLC Separation of Monovalent Inorganic Cations and Anions in HILIC, Cation- and Anion-Exchange Modes on Amaze TCH
Conditions of Experiment
Column: Amaze TCH
Separation Modes: HILIC, cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 73% ACN with 20 mM AmFm pH 2.7
Detection: Corona CAD
Sample: 0.5 mg/ml
Injection: 2 ul
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic anion, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Iodide ion, Potassium ion, Sodium ion, Lithium ion, Bromide ion, Chloride Ion
HPLC UV Analysis of Four Basic Drugs and Six Acidic Counterions on Heritage MA Mixed-Mode Column
Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase, anion-exchange, cation-exclusion
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: ACN/Water/Ammonium Phosphate pH 2.2
Detection: 205 nm
Sample: 0.2-0.5 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Acidic counterion, Amines, Aromatic base, Aromatic compound, Drug, Inorganic anion, Organic acid, Sugar
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Chlorpheniramine, Brompheniramine, Acetic acid, Lactic acid, Iodide ion, Succinic acid, Malic acid, Saccharic acid, Propranolol, Dextromethorphan
HPLC Separation of Sodium and Four Inorganic Anions on Amaze TH Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 40% ACN with gradient of AmFm pH 3 from 30 mM
to 120 mM in 7.5 min, hold 8 min
Detection: ELSD
Sample: 0.5-1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic acid, Inorganic anion, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Phosphate Ion, Chloride Ion, Sodium ion, Iodide ion, Sulfate ion
HPLC Analysis of Seven Anions on Heritage MA Mixed-Mode Column
Application description

Organic and inorganic acids are part of many formulations including pharmaceutical and nutraceuticals. These compounds are very often very polar and acidic, and either require expensive ion-chromatography or the use of ion-pairing reagents. Ion-pairing reagents are not compatible with mass spectrometry. Mixed-mode columns allow the utilization of the ion-pairing reagent attached to the surface of silica gel to retain these anionic compounds. The retention is provided by weak reverse-phase and by a medium or strong anion-exchange mechanism. The retention time is controlled by the amount of ACN, buffer pH and buffer concentration. Stronger and more hydrophobic acids will retain longer, and their elution can be effectively controlled by changing the ionization state at a different pH. This method is compatible with major detection techniques ( (ELSD, CAD, RI, UV and mass spec). This method can be used for the analysis of basic hydrophobic drugs and their acidic counter-ions in one run. Acidic hydrophobic drugs and related impurities can be analyzed on this mixed-mode HPLC column.

Conditions of Experiment
Column: Heritage MA
Separation Modes: anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 60% ACN with 60 mM AmFm pH 3
Detection: ELSD, 50°C
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic anion, Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Phosphate Ion, Bromate Ion, Bromide ion, Chloride Ion, Iodide ion, Trifluoroacetic acid, Perchlorate ion
Simultaneous HPLC Analysis of Sodium Ion and Iodide Ion on Amaze TH Mixed-Mode Column
Conditions of Experiment
Column: Amaze TH
Separation Modes: cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm 5 um, 100A
Mobile Phase: 50% ACN with 20 mM AmAc pH 4
Detection: ELSD
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Inorganic anion, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Iodide ion, Sodium ion