Isoascorbic acid Isoascorbic acid Isoascorbic acid Select Applications HPLC Separation of Isomers of Ascorbic Acid and Ascorbic Acid-2-Phosphate in HILIC/Mixed-Mode on Amaze HD Column HPLC Separation of Isomers of Ascorbic Acid in Reversed-Phase and Anion-Exchange Modes on Coresep SB Column HPLC Separation of Isomers of Ascorbic Acid in Reversed-Phase and Anion-Exchange Modes on Amaze HA Column HPLC Separation of Isomers of Ascorbic Acid in HILIC and Anion-Exclusion Modes on Amaze HD Column HPLC Separation of Isomers of Ascorbic Acid and Ascorbic Acid-2-Phosphate in HILIC/Mixed-Mode on Amaze HD Column Amaze HA PURCHASE LEARN ABOUT Amaze HA Conditions of Experiment Column: Amaze HA Separation Modes: hydrophilic acidic Column Dimenstions: 3.0x100 mm, 3 um, 100A Mobile Phase: from 85% ACN with 30 mM Ammonium Acetate pH 4to 50% ACN with 10 mM Ammonium Acetate pH 4 in 10 min Detection: 280 nm Sample: 0.2 mg/ml Injection: 2 uL Flow rate: 0.6 ml/min Analytes Class of compounds: Aromatic acid, Metabolite, Organic acid, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Isoascorbic acid, Ascorbic acid, Ascorbic acid 2-phosphate HPLC Separation of Isomers of Ascorbic Acid in Reversed-Phase and Anion-Exchange Modes on Coresep SB Column Coresep SB PURCHASE LEARN ABOUT Coresep SB Conditions of Experiment Column: Coresep SB Separation Modes: reversed-phase and anion-exchange Column Dimenstions: 4.6x150 mm, 2.7 um , 100A Mobile Phase: 20% ACN with 50 mM Ammonium phosphate pH 4 Detection: 280 nm Sample: 0.2 mg/ml Injection: 2 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Ascorbic acid, Isoascorbic acid HPLC Separation of Isomers of Ascorbic Acid in Reversed-Phase and Anion-Exchange Modes on Amaze HA Column Amaze HA PURCHASE LEARN ABOUT Amaze HA Conditions of Experiment Column: Amaze HA Separation Modes: reversed-phase and anion-exchange Column Dimenstions: 3.0x100 mm, 3 um, 100A Mobile Phase: 2% ACN with 5 mM Ammonium phosphate pH 4 Detection: 280 nm Sample: 0.2 mg/ml Injection: 2 uL Flow rate: 0.6 ml/min Analytes Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Isoascorbic acid, Ascorbic acid HPLC Separation of Isomers of Ascorbic Acid in HILIC and Anion-Exclusion Modes on Amaze HD Column Amaze HD PURCHASE LEARN ABOUT Amaze HD Conditions of Experiment Column: Amaze HD Separation Modes: HILIC, anion-exchange Column Dimenstions: 3.0x100 mm, 3 um, 100A Mobile Phase: 85% ACN with 20 mM Ammonium Acetate pH 4 Detection: 280 nm Sample: 0.2 mg/ml Injection: 2 uL Flow rate: 0.6 ml/min Analytes Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Isoascorbic acid, Ascorbic acid
