Isoleucine

HPLC Analysis of 20 Amino Acids on Amaze SC Mixed-Mode Column in RP and Cation-exchange Modes
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase cation-exchange
Column Dimenstions: 4.6x250 mm, 5 μm, 100A
Mobile Phase: A: 2% ACN with 0.1% TFA B: 25% ACN with 0.3% TFA from 100% A to 100% B in 20 min, 8 min hold
Detection: Corona CAD
Sample: 0.2 mg/ml
Injection: 5 μL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amino acid, Neurotransmitter, Supplement
Nature of compounds: Acidic, Hydrophilic, Polar, Zwitterionic
Compounds: Aspartic acid, Serine, Asparagine, Cysteine, Glutamic acid, Threonine, Glutamine, Glycine, Alanine, Proline, Valine, Methionine, Tyrosine, Isoleucine, Leucine, Phenylalanine, Lysine, Histidine, Tryptophan, Arginine
HPLC Analysis of 18 Amino Acids on Amaze HD Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 3.0x100 mm, 5 um, 100A
Mobile Phase: 80% ACN with 20 mM AmFm pH 3
Detection: ELSD, 45°C
Sample: 0.5-0.8 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amino acid, Catecholamine, Drug, Neurotransmitter, Supplement
Nature of compounds: Acidic, Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Tryptophan, Phenylalanine, Leucine, Methionine, Tyrosine, Isoleucine, DOPA, Valine, Cysteine, Proline, Alanine, Threonine, Glutamic acid, Glycine, Glutamine, Asparagine, Serine, Aspartic acid