Application description
This UV HPLC method is designed for the precise analysis of isomers of Phthalic Acid using an Amaze HA Mixed-Mode Column. The separation is conducted in both reversed-phase and anion-exchange modes, offering versatility in chromatographic characterization.
Phthalic acid, a precursor in the synthesis of industrial chemicals, including phthalate esters and dyes, exists in three isomeric forms: ortho-phthalic acid, meta-phthalic acid, and para-phthalic acid.
Ortho-phthalic acid, known as 1,2-benzenedicarboxylic acid, features two adjacent carboxylic acid functional groups on the benzene ring. It is a colorless crystalline solid with moderate solubility in water.
Meta-phthalic acid, or 1,3-benzenedicarboxylic acid, has carboxylic acid groups attached to carbon atoms separated by one carbon atom on the benzene ring. It shares similar physical properties with ortho-phthalic acid and is sparingly soluble in water.
Para-phthalic acid, or 1,4-benzenedicarboxylic acid, exhibits carboxylic acid functional groups attached to opposite sides of the benzene ring. It is also a colorless crystalline solid with limited solubility in water.
The Amaze HA Mixed-Mode Column is selected for its dual functionality in reversed-phase and anion-exchange modes. In reversed-phase mode, the column facilitates separation based on differences in hydrophobicity, while in anion-exchange mode, it selectively retains and separates charged species, such as the carboxylate ions of phthalic acid isomers.
By employing this UV HPLC method, precise quantification and characterization of Phthalic Acid isomers can be achieved, facilitating their analysis in various industrial and environmental applications.
Conditions of Experiment
| Column: |
Amaze HA |
| Separation Modes: |
reversed-phase, Anion-exchange |
| Column Dimenstions: |
3x100 mm, 3 um, 100 A |
| Mobile Phase: |
ACN/water/ammonium phosphate pH 2.5 |
| Detection: |
235 nm |
| Sample: |
0.2-0.2 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
0.6 ml/min |
Analytes
| Class of compounds: |
Aromatic acid, Isomer, Organic acid |
| Nature of compounds: |
Acidic, Hydrophilic, Hydrophobic, Polar |
| Compounds: |
Terephthalic acid, Isophthalic acid, Phthalic acid |
