Kanamycin
HPLC Analysis of Eight Aminoglycosides on Amaze TCH Column with LC/MS Compatible Conditions
Conditions of Experiment
Column: | Amaze TCH |
Separation Modes: | HILIC and cation-exchange |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | ACN/water/ammonium formate |
Detection: | ELSD |
Sample: | 0.3 mg/ml |
Injection: | 3 uL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Amines, Aminoglycoside, Antibiotic, Drug |
Nature of compounds: | Basic, Hydrophilic |
Compounds: | Erythromycin, Spectinomycin, Hygromycin B, Streptomycin, Amikacin, Kanamycin, Tobramycin, Neomycin |
HPLC Analysis of Five Aminoglycosides on Amaze TR Column in HILIC and Ion-Exchange Modes
Conditions of Experiment
Column: | Amaze TR |
Separation Modes: | HILIC, cation-exchange |
Column Dimenstions: | 3.0x50 mm, 3 um, 100A |
Mobile Phase: | |
Detection: | ELSD, MS |
Sample: | 0.3-0.5 mg/ml each |
Injection: | 3 uL |
Flow rate: | 0.7 ml/min |
Analytes
Class of compounds: | Amines, Aminoglycoside, Antibiotic, Drug |
Nature of compounds: | Basic, Hydrophilic, Polar |
Compounds: | Erythromycin, Azithromycin, Streptomycin, Kanamycin, Gentamycin |