HPLC Separation of Acidic Drugs Naproxen, Ketoprofen, Ibuprofen and Basic Counterion Sodium
Conditions of Experiment
Column: Amaze TR
Separation Modes: Reversed-phase, cation-exchange, anion-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 70% ACN with 10 mM AmAc pH 5
Detection: UV 275 nm ELSD
Sample: 0.5 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Class of compounds: Aromatic acid, Drug, Inorganic cation
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Sodium ion, Naproxen, Ketoprofen, Ibuprofen
HPLC Analysis of Drug Ketoprofen on Heritage C18 Column According to US Pharmacopeia
Conditions of Experiment
Column: Heritage С18
Separation Modes: reversed-phase
Column Dimenstions: 4.6 x 250 mm, 5 um, 100A
Mobile Phase: H2O/MeCN/NaHPO4 pH 3.5 –60/38/2
Detection: UV 230 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 mL/min
Class of compounds: Aromatic acid, Drug
Nature of compounds: Acidic, Hydrophobic
Compounds: Ketoprofen