Lactose

HPLC Analysis of 11 Sugars in HILIC Mode on Amaze HD Mixed-Mode Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 4.6x250 mm, 5 μm, 100A
Mobile Phase: ACN/Water-92/8
Detection: Corona CAD
Sample: 1 mg/ml
Injection: 3 μL
Flow rate: 1 ml/min
Analytes
Class of compounds: Sugar
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Xylose, Fructose, Glucose, Mannose, Galactose, Maltitol, Sucrose, Sorbitol, Maltose, Lactose, Lactulose
HPLC Analysis of Six Sugars in HILIC Mode on Coresep N Mixed-Mode Column
Conditions of Experiment
Column: Coresep N
Separation Modes: HILIC
Column Dimenstions: 4.6x150 mm, 2.7 μm, 90A
Mobile Phase: ACN/water – 90/10
Detection: Corona CAD
Sample: 1 mg/ml
Injection: 3 μL
Flow rate: 1 ml/min
Analytes
Class of compounds: Sugar
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Xylose, Glucose, Sorbitol, Sucrose, Maltose, Lactose
HPLC Analysis of Sugars in HILIC Mode on Amaze HD Mixed-Mode Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 4.6x150 mm, 5 μm, 100A
Mobile Phase: ACN/Formic acid – 95/5
Detection: Corona CAD
Sample: 1 mg/ml
Injection: 3 μL
Flow rate: 1 ml/min
Analytes
Class of compounds: Sugar
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Xylose, Glucose, Maltitol, Sucrose, Lactose
HPLC Analysis of Five Sugars on Amaze SC Mixed-Mode Column in HILIC Mode
Conditions of Experiment
Column: Amaze SC
Separation Modes: HILIC
Column Dimenstions: 4.6x250 mm, 5 μm, 100A
Mobile Phase: A: 98% ACN with 0.02% TFA B: 85% ACN with 0.1% TFA from 100% A to 100% B in 12 min and 3 min hold
Detection: Corona CAD
Sample: approx 0.5 mg/ml
Injection: 3 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Sugar
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Xylose, Glucose, Maltitol, Sucrose, Lactose
HPLC Analysis of Sugars in HILIC Mode on Coresep N Mixed-Mode Column
Conditions of Experiment
Column: Coresep N
Separation Modes: HILIC
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: ACN/water/formic acid
Detection: Corona CAD
Sample: 0.5 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Sugar
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Xylose, Glucose, Sorbitol, Sucrose, Maltose, Lactose
HPLC Analysis of Sugars in HILIC Mode on Amaze HD Mixed-Mode Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: ACN/water/formic acid
Detection: Corona CAD
Sample: 0.5 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Sugar
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Xylose, Glucose, Maltitol, Sucrose, Lactose