Lactose
Lactose
Lactose
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HPLC Separation of Mono-, Di- and Trisaccharides on Amaze HD HILIC Mixed-Mode Column
HPLC Analysis of 11 Sugars in HILIC Mode on Amaze HD Mixed-Mode Column
HPLC Analysis of Six Sugars in HILIC Mode on Coresep N Mixed-Mode Column
HPLC Analysis of Sugars in HILIC Mode on Amaze HD Mixed-Mode Column
HPLC Analysis of Five Sugars on Amaze SC Mixed-Mode Column in HILIC Mode
HPLC Analysis of Sugars in HILIC Mode on Coresep N Mixed-Mode Column
HPLC Analysis of Sugars in HILIC Mode on Amaze HD Mixed-Mode Column
HPLC Separation of Mono-, Di- and Trisaccharides on Amaze HD HILIC Mixed-Mode Column
Conditions of Experiment
Column: | Amaze HD |
Separation Modes: | HILIC |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | ACN/water/AmAc pH 4 |
Detection: | Corona CAD |
Sample: | 1 mg/ml |
Injection: | 2 uL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Sugar |
Nature of compounds: | Basic, Hydrophilic, Neutral, Polar |
Compounds: | Xylose, Fructose, Glucose, Sucrose, Maltose, Lactose, Melezitose, Raffinose |
HPLC Analysis of 11 Sugars in HILIC Mode on Amaze HD Mixed-Mode Column
Conditions of Experiment
Column: | Amaze HD |
Separation Modes: | HILIC |
Column Dimenstions: | 4.6x250 mm, 5 μm, 100A |
Mobile Phase: | ACN/Water-92/8 |
Detection: | Corona CAD |
Sample: | 1 mg/ml |
Injection: | 3 μL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Sugar |
Nature of compounds: | Basic, Hydrophilic, Neutral, Polar |
Compounds: | Xylose, Fructose, Glucose, Mannose, Galactose, Maltitol, Sucrose, Sorbitol, Maltose, Lactose, Lactulose |
HPLC Analysis of Six Sugars in HILIC Mode on Coresep N Mixed-Mode Column
Conditions of Experiment
Column: | Coresep N |
Separation Modes: | HILIC |
Column Dimenstions: | 4.6x150 mm, 2.7 μm, 90A |
Mobile Phase: | ACN/water – 90/10 |
Detection: | Corona CAD |
Sample: | 1 mg/ml |
Injection: | 3 μL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Sugar |
Nature of compounds: | Basic, Hydrophilic, Neutral, Polar |
Compounds: | Xylose, Glucose, Sorbitol, Sucrose, Maltose, Lactose |
HPLC Analysis of Sugars in HILIC Mode on Amaze HD Mixed-Mode Column
Conditions of Experiment
Column: | Amaze HD |
Separation Modes: | HILIC |
Column Dimenstions: | 4.6x150 mm, 5 μm, 100A |
Mobile Phase: | ACN/Formic acid – 95/5 |
Detection: | Corona CAD |
Sample: | 1 mg/ml |
Injection: | 3 μL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Sugar |
Nature of compounds: | Basic, Hydrophilic, Neutral, Polar |
Compounds: | Xylose, Glucose, Maltitol, Sucrose, Lactose |
HPLC Analysis of Five Sugars on Amaze SC Mixed-Mode Column in HILIC Mode
Conditions of Experiment
Column: | Amaze SC |
Separation Modes: | HILIC |
Column Dimenstions: | 4.6x250 mm, 5 μm, 100A |
Mobile Phase: | A: 98% ACN with 0.02% TFA B: 85% ACN with 0.1% TFA from 100% A to 100% B in 12 min and 3 min hold |
Detection: | Corona CAD |
Sample: | approx 0.5 mg/ml |
Injection: | 3 μL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Sugar |
Nature of compounds: | Basic, Hydrophilic, Neutral, Polar |
Compounds: | Xylose, Glucose, Maltitol, Sucrose, Lactose |
HPLC Analysis of Sugars in HILIC Mode on Coresep N Mixed-Mode Column
Conditions of Experiment
Column: | Coresep N |
Separation Modes: | HILIC |
Column Dimenstions: | 4.6x150 mm, 5 um, 100A |
Mobile Phase: | ACN/water/formic acid |
Detection: | Corona CAD |
Sample: | 0.5 mg/ml |
Injection: | 2 uL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Sugar |
Nature of compounds: | Basic, Hydrophilic, Neutral, Polar |
Compounds: | Xylose, Glucose, Sorbitol, Sucrose, Maltose, Lactose |
HPLC Analysis of Sugars in HILIC Mode on Amaze HD Mixed-Mode Column
Conditions of Experiment
Column: | Amaze HD |
Separation Modes: | HILIC |
Column Dimenstions: | 4.6x150 mm, 5 um, 100A |
Mobile Phase: | ACN/water/formic acid |
Detection: | Corona CAD |
Sample: | 0.5 mg/ml |
Injection: | 2 uL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Sugar |
Nature of compounds: | Basic, Hydrophilic, Neutral, Polar |
Compounds: | Xylose, Glucose, Maltitol, Sucrose, Lactose |