Maleic acid

Simultaneous HPLC Analysis of Brompheniramine and Maleic Acid Counter-Ion on Heritage MA Column
Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase, cation-exclusion, anion-exchange
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: ACN/water/ammonium formate
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic base, Drug, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Brompheniramine, Maleic acid
HPLC Analysis of Six Organic Acids on Heritage MA Mixed-Mode Column
Conditions of Experiment
Column: Heritage MA
Separation Modes: reveresed-phase, anion-exchange
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: 35% ACN with 50 mM AmFm pH 3
Detection: UV 255 nm
Sample: 0.2 mg/ml
Injection: 5 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic acid, Organic acid, Preservative
Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Polar
Compounds: Gallic acid, 4-Hydroxybenzoic acid, Caffeic acid, Mandelic acid, Maleic acid, Atrolactic acid
HPLC Analysis of Hydrophilic Acids on Coresep SB Column in Reversed-Phase and Anion-Exchange Modes
Application description

Mixture of hydrophilic acidic compounds are separated in reversed-phase anion-exchange mode on core-shell mixed-mode column. Method is robust, reliable and fast and can be used for analysis of underivatized acids without ion-pairing reagent.

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: 50% ACN with 50 mM sodium phosphate (monobasic) pH 3
Detection: UV 230 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 0.5 mg/ml
Analytes
Class of compounds: Aromatic acid, Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Benzoic acid, Maleic acid, Fumaric acid