HPLC Analysis of Sugar Alcohols and Sugars on Amaze HD Mixed-Mode Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 4.6x50 mm, 5 um, 100A
Mobile Phase: 80% ACN with 20 mM AmFm pH 3
Detection: ELSD 40°C
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Sugar
Nature of compounds: Hydrophilic, Neutral, Polar
Compounds: Xylitol, Mannitol, Sucrose, Maltose, Melezitose