Mandelic acid Mandelic acid Mandelic acid Select Applications HPLC Separation of 15 Organic Acids on Amaze HA Mixed-Mode Column with a Triple Gradient HPLC Analysis of 6 Aromatic Acids and Alcohols on Amaze Aromatic Stationary Phases HPLC Analysis of Aromatic Carboxylic Acids on Amaze TR Mixed-Mode Column HPLC Analysis of Six Organic Acids on Heritage MA Mixed-Mode Column HPLC Separation of Four Aromatic Acids on Heritage MA Mixed-Mode Column HPLC Separation of 15 Organic Acids on Amaze HA Mixed-Mode Column with a Triple Gradient Amaze HA PURCHASE LEARN ABOUT Amaze HA Conditions of Experiment Column: Amaze HA Separation Modes: reveresed-phase , anion-exchange Column Dimenstions: 4.6x100, 3 um, 100A Mobile Phase: "A: 2% ACN with 40 mM ammonium phosphate pH 4.0 B: 20% ACN with 100 mM ammonium phosphate pH 2.0 gradient from 100% A to 100% B in 15 minutes, 5 minutes hold " Detection: 205 nm Sample: 0.2-0.5 mg/ml Injection: 2 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Isomer, Organic acid, Organic compound, Preservative, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Neutral, Polar Compounds: Acetic acid, Ascorbic acid, Formic acid, Succinic acid, Citric acid, Malonic acid, Fumaric acid, Mandelic acid, Oxalic acid, Terephthalic acid, Benzoic acid, Maleic acid, Phthalic acid, Benzenesulfonic acid, p-Toluenesulfonic acid HPLC Analysis of 6 Aromatic Acids and Alcohols on Amaze Aromatic Stationary Phases Application description Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C20 columns. Amaze SPF PURCHASE LEARN ABOUT Amaze SPF Conditions of Experiment Column: Amaze SPF Separation Modes: reversed-phase, pi-pi Column Dimenstions: 4.6x150 mm, 5 μm, 100A Mobile Phase: 35% ACN with 20 mM AmAc pH 4.5 Detection: UV 235 nm Sample: 1 mg/ml Injection: 3 μL Flow rate: 1 ml/min Analytes Class of compounds: Amines, Aromatic acid, Aromatic base, Aromatic compound, Organic acid, Organic compound, Preservative Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar Compounds: 4-Aminophenol, Mandelic acid, 4-Hydroxybenzoic acid, Phenol, Benzoic acid, Methylparaben HPLC Analysis of Aromatic Carboxylic Acids on Amaze TR Mixed-Mode Column Application description Aromatic organic acids are used as building blocks in chemistry, additives in various formulations and counter-ions in drug production. In most of the cases, these acids are hydrophilic and acidic in nature with limited retention in reversed-phase chromatography. We have developed a simple, robust and isocratic method to separate mandelic, gallic, phthalic and 4-hydroxybenzoic acids on the Amaze TR HPLC column. All four acids are retained and separated by combination of weak reversed-phase and weak anion-exchange mechanisms. The baseline separation is achieved within 5 minutes. This method can be used for the analysis of hydrophilic and hydrophobic organic and inorganic acids with LC/MS-compatible conditions. Amaze TR PURCHASE LEARN ABOUT Amaze TR Conditions of Experiment Column: Amaze TR Separation Modes: reversed-phase, anion-exchange Column Dimenstions: 4.6x50 mm, 3 um, 100A Mobile Phase: 20% ACN with 20 mM AmFm pH 3 Detection: UV 255 nm Sample: 0.3 mg/ml Injection: 3 ul Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Isomer, Organic acid, Preservative Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Polar Compounds: Mandelic acid, Gallic acid, Phthalic acid, 4-Hydroxybenzoic acid HPLC Analysis of Six Organic Acids on Heritage MA Mixed-Mode Column Heritage MA PURCHASE LEARN ABOUT Heritage MA Conditions of Experiment Column: Heritage MA Separation Modes: reveresed-phase, anion-exchange Column Dimenstions: 4.6 x 150 mm, 5 um, 100A Mobile Phase: 35% ACN with 50 mM AmFm pH 3 Detection: UV 255 nm Sample: 0.2 mg/ml Injection: 5 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Organic acid, Preservative Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Polar Compounds: Gallic acid, 4-Hydroxybenzoic acid, Caffeic acid, Mandelic acid, Maleic acid, Atrolactic acid HPLC Separation of Four Aromatic Acids on Heritage MA Mixed-Mode Column Heritage MA PURCHASE LEARN ABOUT Heritage MA Conditions of Experiment Column: Heritage MA Separation Modes: reversed-phase and anion-exchange Column Dimenstions: 4.6 x 150 mm Mobile Phase: 20% ACN with 100 mM AmFm pH 3 Detection: UV 255 nm Sample: 0.3 mg/ml Injection: 1 uL Flow rate: 1 mL/min Analytes Class of compounds: Aromatic acid, Catecholamine, Neurotransmitter, Organic acid Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Vanillylmandelic Acid, Mandelic acid, Atrolactic acid, α-Methoxyphenylacetic acid
