Mannitol

HPLC Analysis of Sugar Alcohols and Sugars on Amaze HD Mixed-Mode Column
Application description

Sugar alcohols and sugars are carbohydrates which are used in food and can be found in fruits, vegetables and other naturally occurring products. Simple sugars include monosaccharides like glucose, fructose and galactose. Sugar alcohols are derived from sugars and are occurring naturally, or synthesized by hydrogenation of sugars. Both sugars and sugar alcohols are polar with no chromophores. The most common approach for the analysis of sugars and sugar alcohols is HILIC chromatography. These hydrophilic compounds are retained by a polar mechanism on a polar stationary phase like Amaze HD. This column has a very polar group on the surface of silica gel which allows the use of less ACN for the retention of sugars and sugar alcohols. This method and the column can be used for the analysis of sugars and sugar alcohols in fruits, veggies, juices and other beverages. The Amaze HD is compatible with 100% ACN and 100% water. ELSD, CAD, RI or MS are required to retain and monitor sugars.

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 4.6x50 mm, 5 um, 100A
Mobile Phase: 80% ACN with 20 mM AmFm pH 3
Detection: ELSD 40°C
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Sugar
Nature of compounds: Hydrophilic, Neutral, Polar
Compounds: Xylitol, Mannitol, Sucrose, Maltose, Melezitose
HPLC Analysis of Etidronic Acid, Citric Acid and Mannitol on Amaze HD Mixed-Mode Column
Application description

Aromatic organic acids are used as building blocks in chemistry, additives in various formulations and counter-ions in drug production. In most of the cases, these acids are hydrophilic and acidic in nature with limited retention in reversed-phase chromatography. We have developed a simple, robust and isocratic method to separate mandelic, gallic, phthalic and 4-hydroxybenzoic acids on the Amaze TR HPLC column. All four acids are retained and separated by combination of weak reversed-phase and weak anion-exchange mechanisms. The baseline separation is achieved within 5 minutes. This method can be used for the analysis of hydrophilic and hydrophobic organic and inorganic acids with LC/MS-compatible conditions.

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, anion-exchange
Column Dimenstions: 4.6x50 mm, 5 um, 100A
Mobile Phase: 80% ACN with 20 mM AmFm pH 3
Detection: ELSD, 50°C
Sample: 2 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Drug, Organic acid, Preservative, Sugar
Nature of compounds: Acidic, Hydrophilic, Neutral, Polar
Compounds: Citric acid, Etidronic acid, Mannitol
HPLC Analysis of Ibandronic Acid, Citric Acid and Mannitol on Amaze HD Mixed-Mode Column
Application description

Mannitol, ibandronic and citric acids are part of the formulation to reduce bone resorption, strengthen bones and treat Osteoporosis. Etidronic acid also prevents bone calcification. Citric acid is used as a preservative in the formulation. All three compounds are very polar in nature with citric and etidronic acids being acidic and mannitol being neutral. All three are not retained in RP chromatography. In HILIC, citric acid and etidronic acid can elute with a poor peak shape due to unspecified secondary interactions. We have developed a robust HILIC/anion-exclusion method for separation of these polar compounds. This method is compatible with major detection techniques for such compounds with no UV activity. (ELSD, CAD, RI and MS). The method can be used for analysis of polar acidic, basic and neutral compounds in HILIC, cation-exchange and anion-exclusion modes. The retention time is controlled by the amount of acetonitrile, buffer pH and buffer concentration. It is recommended to use salt buffer to achieve better retention and elution control.

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, anion-exchange
Column Dimenstions: 4.6x50 mm, 5 um, 100A
Mobile Phase: ACN/water/Buffer
Detection: ELSD, 50°C
Sample: 2 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Drug, Organic acid, Preservative, Sugar
Nature of compounds: Acidic, Hydrophilic, Neutral, Polar
Compounds: Ibandronic acid, Citric acid, Mannitol