MCPA

HPLC Analysis of Herbicide MCPA on Coresep 100 Mixed-Mode Column
Application description

MCPA or (2-methyl-4-chlorophenoxyacetic acid is a powerful and selective phenoxy herbicide. It is used to control broadleaf weeds. It is also used in the chemical industry due to the ability to form conjugated complexes with metals. MCPA is hydrophobic and acidic and retains on Coresep 100 mixed-mode column by the combination of reversed-phase and anion-exclusion mechanisms. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various herbicides with LC/MS compatible conditions. Core-shell mixed-mode chromatography offers a synergy of unique selectivity of mixed-mode stationary phases and high efficiency of core-shell particles.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and anion-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Analytes
Class of compounds: Aromatic acid, Herbicide
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: MCPA
Baseline HPLC Separation of 22 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column
Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase, cation-exchange, anion-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold
Detection: UV 275 nm
Sample: 0.1-0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Analytes
Class of compounds: Amino acid, Antibiotic, Aromatic acid, Aromatic base, Aromatic compound, Drug, Fungicide, Herbicide, Insecticide, Pesticide, Pyridine
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic
Compounds: Clopyralid (herbicide), Sulfadiazine, Sulfamethazine, Cefepime (antibiotic), Metribuzin, Bromacil, 2,4-D, MCPA, Thiacloprid (insecticide), 2,4,5-T, Diphenamid, Captan (fungicide), 2,4-DB, Triadimefon, Azinphos Ethyl, Diazinon, Chlorpyrifos Methyl, Phoxim, Teramisol (antibiotic), Benfluralin (herbicide), Dicofol, Hexachlorobenzene
HPLC Analysis of Herbicide MCPA on Heritage MA Mixed-Mode Column
Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 4.6 x 50 mm
Mobile Phase: 60% ACN with 80 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1 mL/min
Analytes
Class of compounds: Aromatic acid, Herbicide
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: MCPA