Application description
Metformin is an antidiabetic drug that helps with reducing blood glucose levels and improves insulin sensitivity. It is used to control Type 2 diabetes. Metformin is a guanidine type of compound which is polar and basic in nature. The addition of ion-pairing reagents is required to achieve retention time in RP chromatography. Metformin was analyzed without the use of an ion-pairing reagent on the Coresep 100 mixed-mode column. The retention time of metformin is controlled by an ion-exchange mechanism and can be adjusted by varying the buffer concentration and buffer pH. The amount of ACN will have a limited effect on RP retention but affects ionization state of stationary phase and metformin. Various mobile phases can be used with the Coresep 100 column to accommodate numerous detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection. This method and the mixed-mode HPLC column can be used for the analysis of metformin and other hydrophilic/basic compounds without ion-pairing reagents. Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency.
Conditions of Experiment
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase and cation-exchange |
| Column Dimenstions: |
3.2 x 100 mm, 2.7 um, 90A |
| Mobile Phase: |
MeCN 20% with 0.12% phosphoric acid |
| Detection: |
UV 275 nm |
| Sample: |
0.1-0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.6 mL/min |
Analytes
| Class of compounds: |
Drug |
| Nature of compounds: |
Basic, Hydrophilic, Polar |
| Compounds: |
Metformin |
