HPLC Analysis of Fungicide Tebuconazole on Coresep 100 Mixed-Mode Column
Application description

Tebuconazole is triazole fungicide used in agriculture to treat pathogenic fungi. Tebuconazole is hydrophobic and slightly basic. It retains on Coresep 100 column by combination of strong reversed-phase and weak cation-exchange mechanisms. This robust method is compatible with major detection techniques including mass spectrometry.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min with 3 min hold
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Class of compounds: Herbicide, Pesticide
Nature of compounds: Hydrophobic, Neutral
Compounds: Methiocarb