Methionine Methionine Methionine Select Applications HPLC Analysis of 20 Amino Acids on Amaze SC Mixed-Mode Column in RP and Cation-exchange Modes HPLC Analysis of 18 Amino Acids on Amaze HD Column HPLC Separation of Underivatized Amino Acids in Buffer-Less Mode on Coresep 100 Core-Shell Mixed-Mode Column HPLC Analysis of 20 Amino Acids on Amaze SC Mixed-Mode Column in RP and Cation-exchange Modes Amaze SC PURCHASE LEARN ABOUT Amaze SC Conditions of Experiment Column: Amaze SC Separation Modes: reversed-phase cation-exchange Column Dimenstions: 4.6x250 mm, 5 μm, 100A Mobile Phase: A: 2% ACN with 0.1% TFA B: 25% ACN with 0.3% TFA from 100% A to 100% B in 20 min, 8 min hold Detection: Corona CAD Sample: 0.2 mg/ml Injection: 5 μL Flow rate: 1 ml/min Analytes Class of compounds: Amino acid, Neurotransmitter, Supplement Nature of compounds: Acidic, Hydrophilic, Polar, Zwitterionic Compounds: Aspartic acid, Serine, Asparagine, Cysteine, Glutamic acid, Threonine, Glutamine, Glycine, Alanine, Proline, Valine, Methionine, Tyrosine, Isoleucine, Leucine, Phenylalanine, Lysine, Histidine, Tryptophan, Arginine HPLC Analysis of 18 Amino Acids on Amaze HD Column Amaze HD PURCHASE LEARN ABOUT Amaze HD Conditions of Experiment Column: Amaze HD Separation Modes: HILIC, cation-exchange Column Dimenstions: 3.0x100 mm, 5 um, 100A Mobile Phase: 80% ACN with 20 mM AmFm pH 3 Detection: ELSD, 45°C Sample: 0.5-0.8 mg/ml Injection: 3 uL Flow rate: 0.6 ml/min Analytes Class of compounds: Amino acid, Catecholamine, Drug, Neurotransmitter, Supplement Nature of compounds: Acidic, Basic, Hydrophilic, Polar, Zwitterionic Compounds: Tryptophan, Phenylalanine, Leucine, Methionine, Tyrosine, Isoleucine, DOPA, Valine, Cysteine, Proline, Alanine, Threonine, Glutamic acid, Glycine, Glutamine, Asparagine, Serine, Aspartic acid HPLC Separation of Underivatized Amino Acids in Buffer-Less Mode on Coresep 100 Core-Shell Mixed-Mode Column Application description Amino acids are very polar compounds which are used as a building blocks in pharmaceutical industry. They are widely used as supplements and food additives. Depending on the pH of the mobile phase they can exist in acidic, basic or zwitter-ionic form. At pH below 3, amino acids are basic in nature and have the highest hydrophobicity. Within pH of 3 to 7 amino acids are in zwitter-ionic form where they are the most hydrophilic. There is no mechanism of retention on reversed-phase column and amino acids are not retained. Bufferless ion-separation (BLIS) was introduced by SIELC as a way to retain and analyze amino acids in reversed-phase cation-exchange mode. This mode allows to analyze amino acids as zwitter-ions without any ions/buffers in the mobile phase. The method was adopted for analysis of amino acids on core-shell mixed-mode columns. A similar approach on a reversed-phase core-shell column results in distorted peaks and no separation or significant retention to achieve baseline separation. Coresep 100 PURCHASE LEARN ABOUT Coresep 100 Conditions of Experiment Column: Coresep 100 Separation Modes: reversed-phase and cation-exchange Column Dimenstions: 3.2 x 50 mm, 2.7 um, 90A Mobile Phase: 20% ACN with no additives Detection: UV 205 nm, ELSD Sample: 0.3 mg/ml Injection: 1 uL Flow rate: 1 ml/min Analytes Class of compounds: Amino acid, Supplement Nature of compounds: Hydrophilic, Polar, Zwitterionic Compounds: Methionine, Tyrosine, Phenylalanine
